|
|
| Line 32: |
Line 32: |
| | <div class="experience"> | | <div class="experience"> |
| | *Keio Collection | | *Keio Collection |
| - | **[S20] JW4251: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S20 S20] JW4251: LB Kan1000x 200rpm 37°C |
| - | **[S21] JN0729: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S21 S21] JN0729: LB Kan1000x 200rpm 37°C |
| - | **[S22] JN0728: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S22 S22] JN0728: LB Kan1000x 200rpm 37°C |
| - | **[S23] JN0727: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S23 S23] JN0727: LB Kan1000x 200rpm 37°C |
| - | **[S24] JN0940: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S24 S24] JN0940: LB Kan1000x 200rpm 37°C |
| - | **[S24] JN0940: LB Kan1000x 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S24 S24] JN0940: LB Kan1000x 37°C |
| - | **[S25] JW5100: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S25 S25] JW5100: LB Kan1000x 200rpm 37°C |
| - | **[S25] JW5100: LB Kan1000x 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S25 S25] JW5100: LB Kan1000x 37°C |
| - | **[S26] JN5181: LB Kan1000x 200rpm 37°C | + | **[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S26 S26] JN5181: LB Kan1000x 200rpm 37°C |
| - | *[S27] FF64: LB Tet1000x 200rpm 37°C | + | *[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S27 S27] FF64: LB Tet1000x 200rpm 37°C |
| - | *[S28] NEC280: LB 200rpm 37°C | + | *[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S28 S28] NEC280: LB 200rpm 37°C |
| - | *[S5] MG4: LB Kan NaCl20% 200rpm 37°C | + | *[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S5 S5] MG4: LB Kan NaCl20% 200rpm 37°C |
| - | *[S6] DH5α: LB 200rpm 37°C | + | *[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S6 S6] DH5α: LB 200rpm 37°C |
| | <br> | | <br> |
| - | *[S24] JN0940: LBagar plate with Kan1000x 37°C | + | *[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S24 S24] JN0940: LBagar plate with Kan1000x 37°C |
| - | *[S25] JW5100: LBagar plate with Kan1000x 37°C | + | *[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S25 S25] JW5100: LBagar plate with Kan1000x 37°C |
| | </div> | | </div> |
| | <div class="guillaume"> | | <div class="guillaume"> |
NoteBook
| June
|
| M | T | W | T | F | S | S
|
| [http://2009.igem.org/Team:Paris/1_June_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_June_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/3_June_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_June_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_June_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/6_June_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_June_2009&action=edit 7]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/8_June_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_June_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_June_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_June_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_June_2009&action=edit 12]
| [http://2009.igem.org/Team:Paris/13_June_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/14_June_2009&action=edit 14]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/15_June_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/16_June_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/17_June_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/18_June_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/19_June_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/20_June_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/21_June_2009&action=edit 21]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/22_June_2009&action=edit 22]
| [http://2009.igem.org/Team:Paris/23_June_2009 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/24_June_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/25_June_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/26_June_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/27_June_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/28_June_2009&action=edit 28]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/29_June_2009&action=edit 29]
| [http://2009.igem.org/Team:Paris/30_June_2009 30]
|
|
| July
|
| M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/1_July_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_July_2009&action=edit 2]
| [http://2009.igem.org/Team:Paris/3_July_2009 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_July_2009&action=edit 5]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/6_July_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_July_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/8_July_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_July_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_July_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_July_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_July_2009&action=edit 12]
|
| [http://2009.igem.org/Team:Paris/13_July_2009 13]
| [http://2009.igem.org/Team:Paris/14_July_2009 14]
| [http://2009.igem.org/Team:Paris/15_July_2009 15]
| [http://2009.igem.org/Team:Paris/16_July_2009 16]
| [http://2009.igem.org/Team:Paris/17_July_2009 17]
| [http://2009.igem.org/Team:Paris/18_July_2009 18]
| [http://2009.igem.org/Team:Paris/19_July_2009 19]
|
| [http://2009.igem.org/Team:Paris/20_July_2009 20]
| [http://2009.igem.org/Team:Paris/21_July_2009 21]
| [http://2009.igem.org/Team:Paris/22_July_2009 22]
| [http://2009.igem.org/Team:Paris/23_July_2009 23]
| [http://2009.igem.org/Team:Paris/24_July_2009 24]
| [http://2009.igem.org/Team:Paris/25_July_2009 25]
| [http://2009.igem.org/Team:Paris/26_July_2009 26]
|
| [http://2009.igem.org/Team:Paris/27_July_2009 27]
| [http://2009.igem.org/Team:Paris/28_July_2009 28]
| [http://2009.igem.org/Team:Paris/29_July_2009 29]
| [http://2009.igem.org/Team:Paris/30_July_2009 30]
| [http://2009.igem.org/Team:Paris/31_July_2009 31]
|
|
| August
|
| M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/Team:Paris/1_August_2009 1]
| [http://2009.igem.org/Team:Paris/2_August_2009 2]
|
| [http://2009.igem.org/Team:Paris/3_August_2009 3]
| [http://2009.igem.org/Team:Paris/4_August_2009 4]
| [http://2009.igem.org/Team:Paris/5_August_2009 5]
| [http://2009.igem.org/Team:Paris/6_August_2009 6]
| [http://2009.igem.org/Team:Paris/7_August_2009 7]
| [http://2009.igem.org/Team:Paris/8_August_2009 8]
| [http://2009.igem.org/Team:Paris/9_August_2009 9]
|
| [http://2009.igem.org/Team:Paris/10_August_2009 10]
| [http://2009.igem.org/Team:Paris/11_August_2009 11]
| [http://2009.igem.org/Team:Paris/12_August_2009 12]
| [http://2009.igem.org/Team:Paris/13_August_2009 13]
| [http://2009.igem.org/Team:Paris/14_August_2009 14]
| [http://2009.igem.org/Team:Paris/15_August_2009 15]
| [http://2009.igem.org/Team:Paris/16_August_2009 16]
|
| [http://2009.igem.org/Team:Paris/17_August_2009 17]
| [http://2009.igem.org/Team:Paris/18_August_2009 18]
| [http://2009.igem.org/Team:Paris/19_August_2009 19]
| [http://2009.igem.org/Team:Paris/20_August_2009 20]
| [http://2009.igem.org/Team:Paris/21_August_2009 21]
| [http://2009.igem.org/Team:Paris/22_August_2009 22]
| [http://2009.igem.org/Team:Paris/23_August_2009 23]
|
| [http://2009.igem.org/Team:Paris/24_August_2009 24]
| [http://2009.igem.org/Team:Paris/25_August_2009 25]
| [http://2009.igem.org/Team:Paris/26_August_2009 26]
| [http://2009.igem.org/Team:Paris/27_August_2009 27]
| [http://2009.igem.org/Team:Paris/28_August_2009 28]
| [http://2009.igem.org/Team:Paris/29_August_2009 29]
| [http://2009.igem.org/Team:Paris/30_August_2009 30]
|
| [http://2009.igem.org/Team:Paris/31_August_2009 31]
|
|
| September
|
| M | T | W | T | F | S | S
|
|
| [http://2009.igem.org/Team:Paris/1_September_2009 1]
| [http://2009.igem.org/Team:Paris/2_September_2009 2]
| [http://2009.igem.org/Team:Paris/3_September_2009 3]
| [http://2009.igem.org/Team:Paris/4_September_2009 4]
| [http://2009.igem.org/Team:Paris/5_September_2009 5]
| [http://2009.igem.org/Team:Paris/6_September_2009 6]
|
| [http://2009.igem.org/Team:Paris/7_September_2009 7]
| [http://2009.igem.org/Team:Paris/8_September_2009 8]
| [http://2009.igem.org/Team:Paris/9_September_2009 9]
| [http://2009.igem.org/Team:Paris/10_September_2009 10]
| [http://2009.igem.org/Team:Paris/11_September_2009 11]
| [http://2009.igem.org/Team:Paris/12_September_2009 12]
| [http://2009.igem.org/Team:Paris/13_September_2009 13]
|
| [http://2009.igem.org/Team:Paris/14_September_2009 14]
| [http://2009.igem.org/Team:Paris/15_September_2009 15]
| [http://2009.igem.org/Team:Paris/16_September_2009 16]
| [http://2009.igem.org/Team:Paris/17_September_2009 17]
| [http://2009.igem.org/Team:Paris/18_September_2009 18]
| [http://2009.igem.org/Team:Paris/19_September_2009 19]
| [http://2009.igem.org/Team:Paris/20_September_2009 20]
|
| [http://2009.igem.org/Team:Paris/21_September_2009 21]
| [http://2009.igem.org/Team:Paris/22_September_2009 22]
| [http://2009.igem.org/Team:Paris/23_September_2009 23]
| [http://2009.igem.org/Team:Paris/24_September_2009 24]
| [http://2009.igem.org/Team:Paris/25_September_2009 25]
| [http://2009.igem.org/Team:Paris/26_September_2009 26]
| [http://2009.igem.org/Team:Paris/27_September_2009 27]
|
| [http://2009.igem.org/Team:Paris/28_September_2009 28]
| [http://2009.igem.org/Team:Paris/29_September_2009 29]
| [http://2009.igem.org/Team:Paris/30_September_2009 30]
|
|
| October
|
| M | T | W | T | F | S | S
|
|
|
|
| [http://2009.igem.org/Team:Paris/1_October_2009 1]
| [http://2009.igem.org/Team:Paris/2_October_2009 2]
| [http://2009.igem.org/Team:Paris/3_October_2009 3]
| [http://2009.igem.org/Team:Paris/4_October_2009 4]
|
| [http://2009.igem.org/Team:Paris/5_October_2009 5]
| [http://2009.igem.org/Team:Paris/6_October_2009 6]
| [http://2009.igem.org/Team:Paris/7_October_2009 7]
| [http://2009.igem.org/Team:Paris/8_October_2009 8]
| [http://2009.igem.org/Team:Paris/9_October_2009 9]
| [http://2009.igem.org/Team:Paris/10_October_2009 10]
| [http://2009.igem.org/Team:Paris/11_October_2009 11]
|
| [http://2009.igem.org/Team:Paris/12_October_2009 12]
| [http://2009.igem.org/Team:Paris/13_October_2009 13]
| [http://2009.igem.org/Team:Paris/14_October_2009 14]
| [http://2009.igem.org/Team:Paris/15_October_2009 15]
| [http://2009.igem.org/Team:Paris/16_October_2009 16]
| [http://2009.igem.org/Team:Paris/17_October_2009 17]
| [http://2009.igem.org/Team:Paris/18_October_2009 18]
|
| [http://2009.igem.org/Team:Paris/19_October_2009 19]
| [http://2009.igem.org/Team:Paris/20_October_2009 20]
| [http://2009.igem.org/Team:Paris/21_October_2009 21]
| [http://2009.igem.org/Team:Paris/22_October_2009 22]
| [http://2009.igem.org/Team:Paris/23_October_2009 23]
| [http://2009.igem.org/Team:Paris/24_October_2009 24]
| [http://2009.igem.org/Team:Paris/25_October_2009 25]
|
| [http://2009.igem.org/Team:Paris/26_October_2009 26]
| [http://2009.igem.org/Team:Paris/27_October_2009 27]
| [http://2009.igem.org/Team:Paris/28_October_2009 28]
| [http://2009.igem.org/Team:Paris/29_October_2009 29]
| [http://2009.igem.org/Team:Paris/30_October_2009 30]
| [http://2009.igem.org/Team:Paris/31_October_2009 31]
|
|
← Yesterday - Tomorrow →
August 4th
Brain work
A lot of meeting today: First on for the modeling explication and application of stochastic model ?
The second one on every parts and each member's work explanation. We still arguing about the travel agency for the Jamboree...Waaaaaaaaa
Lab work
- Keio Collection
- S20 JW4251: LB Kan1000x 200rpm 37°C
- S21 JN0729: LB Kan1000x 200rpm 37°C
- S22 JN0728: LB Kan1000x 200rpm 37°C
- S23 JN0727: LB Kan1000x 200rpm 37°C
- S24 JN0940: LB Kan1000x 200rpm 37°C
- S24 JN0940: LB Kan1000x 37°C
- S25 JW5100: LB Kan1000x 200rpm 37°C
- S25 JW5100: LB Kan1000x 37°C
- S26 JN5181: LB Kan1000x 200rpm 37°C
- S27 FF64: LB Tet1000x 200rpm 37°C
- S28 NEC280: LB 200rpm 37°C
- S5 MG4: LB Kan NaCl20% 200rpm 37°C
- S6 DH5α: LB 200rpm 37°C
- S24 JN0940: LBagar plate with Kan1000x 37°C
- S25 JW5100: LBagar plate with Kan1000x 37°C
- 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
Transformation of precited Biobrick
- 1K1 (pSB1A3) in competent DH5α by RbCl [Protocol]
- Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).
- D1=[P3]Digested=66pb, D2=[P4]Digested=95pb, D3=[P5]Digested=37pb, D4=[P7]Digested=54pb, D5=[P8]Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification)
- P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
- Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
- D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands)
To do list
| Matricule
| TODO
|
| Luc
| SNARE Construction
|
| Romain
| Design oligo Fec A
|
| Charlotte
| design of oligos: FecR, FecA, SNARE?, sequencing oligo
|
| Stoff
| Dev schedule algorithm / BDD
|
| Chris
| modeling : we don't want fu*king oscillations !
|
| Lisa
| Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting
|
| Caroline
| coloration of cells / protocols for the lab: control of the emission of vesicules....
|
| Souf
| oligos desing / Labs
|
| Vicard
| Gel / SNARE
|
| Pierre
| modeling : we don't want fu*king oscillations !
|
| Sylvain
| minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise
|
| Guillaume
| Lab/ g3p
|
← Yesterday - Tomorrow →
"
