Team:UNIPV-Pavia/Freezer

From 2009.igem.org

(Difference between revisions)

Revision as of 09:20, 9 August 2009

Biobrick/ Ligation Name	Description	Creation Date	N° usage	Strain	Vector	Quality Control
J23100(J100) replaced by "J23100 Jun4"	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23101(J101) replaced by "J23101 Jun4"	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23102(J102)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23103(J103)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23104(J104)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23105(J105)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23106(J106)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23110(J110)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23112(J112)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23113(J113)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23114(J114)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23116(J116)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23117(J117)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118) replaced by "J23118 Jun4"	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
E0240(GFP3)	GFP protein generator (RBS-GFP-TT)	May 25	6	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
K131009(E2)	colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)	May 28	1	TOP10	pSB1AK3	sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB)	colE7 lysis gene	May 28	1	TOP10	pSB1A2	sequence ok (MIT)
K124017(lysis21)	Phage Lysis Cassette	May 28	0	TOP10	pSB1AK3	sequence BAD (MIT)
B0015(TT)	Double terminator	May 28	2	TOP10	pSB1AK3	sequence ok (MIT)
I732017 (RBS-lacZ)	RBS-lacZ	May 28	1	TOP10	pSB1A2	long part (MIT)
B0030	RBS 0.6	Jun 4	1	TOP10	pSB1A2	sequence ok (MIT,BMR)
B0031	RBS 0.07	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
B0032	RBS 0.3	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
B0033	RBS 0.01	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
B0034	RBS 1	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
P0440(P04)	RBS-tetR-TT	Jun 4	1	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
R0040(Ptet)	Ptet	Jun 4	1	TOP10	pSB1A2	sequence ok (MIT)
T9002	Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT	Jun 4	3	TOP10	pSB1A3	sequence BAD (MIT) - sequence ok (BMR)
J23100(J100 Jun4)	constitutive promoter	Jun 4	3	TOP10	J61002(Amp)	sequence ok (MIT,BMR)
J23101(J101 Jun4)	constitutive promoter	Jun 4	2	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118 Jun4)	constitutive promoter	Jun 4	3	TOP10	J61002(Amp)	sequence ok (MIT)
A1	J23100(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	5	TOP10	pSB1A2	length ok (PCR)
A2	J23101(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	5	TOP10	pSB1A2	length ok (PCR)
A3-1	I732017-B0015 (RBS-lacZ-TT)	Jun 12	2	TOP10	pSB1AK3	length ok (digestion) sequence ok -long part- (BMR)
A4	P0440-R0040 (RBS-tetR-TT-Ptet)	Jun 11	2	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A5	A4-A3 (RBS-tetR-TT-Ptet-RBS-lacZ-TT)	Jun 19	0	TOP10	pSB1AK3	sequence ok -long part- (BMR)
A6	J23100(Jun 4)-A4 (Pcon-RBS-tetR-TT-Ptet)	Jun 19	1	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
BOL1	J23118-B0034-C0012-B0015 (Pcon-RBS-lacI-TT)	Jun 23	2	TOP10	pSB1A2	sequence ok (BMR)
A7	J23118(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 25	3	TOP10	pSB1A2	length ok (PCR)
R0011(Plac)	Plac	Jun 25	1	TOP10	pSB1A2	sequence ok (MIT)
K112808(ENTERO)	holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)	Jun 25	1	TOP10	pSB1A2	sequence ok -long part- (MIT)
A8	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	2	TOP10	pSB1A2	NON PURE PLASMID! (digestion) but works
A9	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	2	TOP10	pSB1A2	NON PURE PLASMID! (digestion) but works
A10 thrown away	K117000-B0015 (celB-TT)	Jun 30	2	TOP10	pSB1AK3	sequence BAD - repeat ligation
A8pg	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	0	TOP10	pSB1A2	length ok (digestion)
A9pg	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	2	TOP10	pSB1A2	length ok (digestion)
A11-1 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	1	TOP10	pSB1A2	length ok (PCR) sequence MUTATED
A11-3 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	0	TOP10	pSB1A2	length ok (PCR)
A11-6 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	0	TOP10	pSB1A2	length ok (PCR)
F2620	Ptet-RBS-luxR-TT-Plux	Jul 10	1	TOP10	pSB1A2	sequence BAD (MIT, BMR)
A12-2	J23118(Jun 4)-A4	Jul 16	0	TOP10	pSB1A2	length ok (PCR)sequence ok (BMR)
A12-3	J23118(Jun 4)-A4	Jul 16	0	TOP10	pSB1A2	length ok (PCR)sequence ok (BMR)
A14-4 to throw away!	A11-E0240	Jul 16	0	TOP10	pSB1A2	NON PURE PLASMID! (digestion)
MRGENE1	pdc	Jul 17	0	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE1-2	pdc	Jul 17	0	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE1-3	pdc	Jul 17	0	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE2	adhB	Jul 17	0	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
MRGENE2-2	adhB	Jul 17	0	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
MRGENE2-3	adhB	Jul 17	0	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)

@@ Line 42: / Line 42: @@
 		<td align="center" valign="center"><font face="papyrus" color="royalblue"><b>Quality Control</td>
 </tr>
+	  <tr class="in_freezer"><td class="prova">J23100(J100)<br>replaced by "J23100 Jun4"</td>
+		<td class="prova">constitutive promoter</td>
+                <td class="prova">May 25</td>
+		<td class="prova">0</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">J61002(Amp)</td>
+		<td class="prova">sequence ok (MIT)</td>
+            </tr>
 	  <tr class="in_freezer"><td class="prova">J23101(J101)<br>replaced by "J23101 Jun4"</td>
 		<td class="prova">constitutive promoter</td>
@@ Line 285: / Line 293: @@
 		<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">3</td>
+		<td class="prova">5</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 293: / Line 301: @@
 		<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">1</td>
+		<td class="prova">5</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 333: / Line 341: @@
 		<td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td>
                  <td class="prova">Jun 23</td>
-		<td class="prova">1</td>
+		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 341: / Line 349: @@
 		<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
+		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 349: / Line 357: @@
 		<td class="prova">Plac</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">0</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 357: / Line 365: @@
 		<td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">0</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 410: / Line 418: @@
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)<br>sequence MUTATED</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-3</td>
+	  <tr class="in_freezer"><td class="prova">A11-3<br>to throw away!</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
@@ Line 418: / Line 426: @@
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-6</td>
+	  <tr class="in_freezer"><td class="prova">A11-6<br>to throw away!</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
@@ Line 432: / Line 440: @@
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">sequence BAD (MIT)</td>
+		<td class="prova">sequence BAD (MIT, BMR)</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">A12-2</td>
@@ Line 440: / Line 448: @@
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a></td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a>sequence ok (BMR)</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">A12-3</td>
@@ Line 448: / Line 456: @@
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A14-4</td>
+	  <tr class="in_freezer"><td class="prova">A14-4<br>to throw away!</td>
 		<td class="prova">A11-E0240<br></td>
                  <td class="prova">Jul 16</td>