Team:UNIPV-Pavia/Freezer

From 2009.igem.org

(Difference between revisions)

Revision as of 10:32, 9 August 2009

Biobrick/ Ligation Name	Description	Creation Date	N° usage	Strain	Vector	Quality Control
J23100(J100) replaced by "J23100 Jun4"	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23101(J101) replaced by "J23101 Jun4"	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23102(J102)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23103(J103)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23104(J104)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23105(J105)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23106(J106)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23110(J110)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23112(J112)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23113(J113)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23114(J114)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23116(J116)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23117(J117)	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118) replaced by "J23118 Jun4"	constitutive promoter	May 25	0	TOP10	J61002(Amp)	sequence ok (MIT)
E0240(GFP3)	GFP protein generator (RBS-GFP-TT)	May 25	5	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
K131009(E2)	colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)	May 28	1	TOP10	pSB1AK3	sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB)	colE7 lysis gene	May 28	1	TOP10	pSB1A2	sequence ok (MIT)
K124017(lysis21)	Phage Lysis Cassette	May 28	0	TOP10	pSB1AK3	sequence BAD (MIT)
B0015(TT)	Double terminator	May 28	3	TOP10	pSB1AK3	sequence ok (MIT)
I732017 (RBS-lacZ)	RBS-lacZ	May 28	1	TOP10	pSB1A2	long part (MIT)
B0030	RBS 0.6	Jun 4	1	TOP10	pSB1A2	sequence ok (MIT,BMR)
B0031	RBS 0.07	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
B0032	RBS 0.3	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
B0033	RBS 0.01	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
B0034	RBS 1	Jun 4	0	TOP10	pSB1A2	sequence ok (MIT)
P0440(P04)	RBS-tetR-TT	Jun 4	1	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
R0040(Ptet)	Ptet	Jun 4	1	TOP10	pSB1A2	sequence ok (MIT)
T9002	Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT	Jun 4	2	TOP10	pSB1A3	sequence BAD (MIT) - sequence ok (BMR)
J23100(J100 Jun4)	constitutive promoter	Jun 4	3	TOP10	J61002(Amp)	sequence ok (MIT,BMR)
J23101(J101 Jun4)	constitutive promoter	Jun 4	2	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118 Jun4)	constitutive promoter	Jun 4	3	TOP10	J61002(Amp)	sequence ok (MIT)
A1	J23100(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	3	TOP10	pSB1A2	length ok (PCR)
A2	J23101(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	1	TOP10	pSB1A2	length ok (PCR)
A3-1	I732017-B0015 (RBS-lacZ-TT)	Jun 12	2	TOP10	pSB1AK3	length ok (digestion) sequence ok -long part- (BMR)
A4	P0440-R0040 (RBS-tetR-TT-Ptet)	Jun 11	2	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A5	A4-A3 (RBS-tetR-TT-Ptet-RBS-lacZ-TT)	Jun 19	0	TOP10	pSB1AK3	sequence ok -long part- (BMR)
A6	J23100(Jun 4)-A4 (Pcon-RBS-tetR-TT-Ptet)	Jun 19	1	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
BOL1	J23118-B0034-C0012-B0015 (Pcon-RBS-lacI-TT)	Jun 23	3	TOP10	pSB1A2	sequence ok (BMR)
A7	J23118(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 25	1	TOP10	pSB1A2	length ok (PCR)
R0011(Plac)	Plac	Jun 25	1	TOP10	pSB1A2	sequence ok (MIT)
K112808(ENTERO)	holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)	Jun 25	1	TOP10	pSB1A2	sequence ok -long part- (MIT)
A8	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	1	TOP10	pSB1A2	NON PURE PLASMID! (digestion) but works
A9	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	1	TOP10	pSB1A2	NON PURE PLASMID! (digestion) but works
A10 thrown away	K117000-B0015 (celB-TT)	Jun 30	2	TOP10	pSB1AK3	sequence BAD (BMR) - repeat ligation
A8pg	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	1	TOP10	pSB1A2	length ok (digestion)
A9pg	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	1	TOP10	pSB1A2	length ok (digestion)
A11-1 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	1	TOP10	pSB1A2	length ok (PCR) sequence MUTATED
A11-3 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	0	TOP10	pSB1A2	length ok (PCR)
A11-6 to throw away!	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	0	TOP10	pSB1A2	length ok (PCR)
F2620 to throw away!	Ptet-RBS-luxR-TT-Plux	Jul 10	1	TOP10	pSB1A2	sequence BAD (MIT, BMR)
A12-2	J23118(Jun 4)-A4	Jul 16	1	TOP10	pSB1A2	length ok (PCR)sequence ok (BMR)
A12-3	J23118(Jun 4)-A4	Jul 16	1	TOP10	pSB1A2	length ok (PCR)sequence ok (BMR)
A14-4 to throw away!	A11-E0240	Jul 16	0	TOP10	pSB1A2	NON PURE PLASMID! (digestion)
A14pg to throw away!	A11-E0240	Jul 16	1	TOP10	pSB1A2	NON PURE PLASMID! (digestion)
MRGENE1	pdc	Jul 17	0	TOP10	pMK-RQ (Kan)	antibiotic ok length ok (digestion) sequence ok (Mr Gene)
MRGENE1-2	pdc	Jul 17	0	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE1-3	pdc	Jul 17	0	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE2	adhB	Jul 17	0	TOP10	pMA (Amp)	antibiotic ok length ok (digestion) sequence ok (Mr Gene)
MRGENE2-2	adhB	Jul 17	0	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
MRGENE2-3	adhB	Jul 17	0	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
B1-13	B0030-MRGENE1 (RBS-adhB)	Jul 28	1	TOP10	pSB1A2	length ok (digestion) sequence ok (BMR)
B2-5	B0030-MRGENE2 (RBS-adhB)	Jul 23	2	TOP10	pSB1A2	sequence ok (BMR)
B3-5	B1-B0015 (RBS-pdc-TT)	Jul 31	1	TOP10	pSB1AK3	sequence ok (BMR)
B4-2	B2-B0015 (RBS-adhB-TT)	Jul 31	1	TOP10	pSB1AK3	sequence ok (BMR)

@@ Line 157: / Line 157: @@
 		<td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td>
                  <td class="prova">May 25</td>
-		<td class="prova">6</td>
+		<td class="prova">5</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 189: / Line 189: @@
 		<td class="prova">Double terminator</td>
                  <td class="prova">May 28</td>
-		<td class="prova">2</td>
+		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 261: / Line 261: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">3</td>
+		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A3</td>
@@ Line 293: / Line 293: @@
 		<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">5</td>
+		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 301: / Line 301: @@
 		<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">5</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 341: / Line 341: @@
 		<td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td>
                  <td class="prova">Jun 23</td>
-		<td class="prova">2</td>
+		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 349: / Line 349: @@
 		<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">3</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 373: / Line 373: @@
 		<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jun 29</td>
-		<td class="prova">2</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 381: / Line 381: @@
 		<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jun 29</td>
-		<td class="prova">2</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 392: / Line 392: @@
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">sequence BAD - repeat ligation</td>
+		<td class="prova">sequence BAD (BMR) - repeat ligation</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">A8pg</td>
 		<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jul 3</td>
-		<td class="prova">0</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 405: / Line 405: @@
 		<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jul 3</td>
-		<td class="prova">2</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 434: / Line 434: @@
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">F2620</td>
+	  <tr class="in_freezer"><td class="prova">F2620<br>to throw away!</td>
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 10</td>
@@ Line 445: / Line 445: @@
 		<td class="prova">J23118(Jun 4)-A4<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">0</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 453: / Line 453: @@
 		<td class="prova">J23118(Jun 4)-A4<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">0</td>
+		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 465: / Line 465: @@
 		<td class="prova">pSB1A2</td>
 		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a></td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A14pg<br>to throw away!</td>
+		<td class="prova">A11-E0240<br></td>
+                <td class="prova">Jul 16</td>
+		<td class="prova">1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a></td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE1</td>
-		<td class="prova">pdc<br></td>
+		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
 		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
-		<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
+		<td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a><br>sequence ok (Mr Gene)</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE1-2</td>
-		<td class="prova">pdc<br></td>
+		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
 		<td class="prova">0</td>
@@ Line 483: / Line 491: @@
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE1-3</td>
-		<td class="prova">pdc<br></td>
+		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
 		<td class="prova">0</td>
@@ Line 491: / Line 499: @@
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE2</td>
-		<td class="prova">adhB<br></td>
+		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
 		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
-		<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
+		<td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a><br>sequence ok (Mr Gene)</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE2-2</td>
-		<td class="prova">adhB<br></td>
+		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
 		<td class="prova">0</td>
@@ Line 507: / Line 515: @@
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE2-3</td>
-		<td class="prova">adhB<br></td>
+		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
 		<td class="prova">0</td>
@@ Line 513: / Line 521: @@
 		<td class="prova">pMA<br>(Amp)</td>
 		<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B1-13</td>
+		<td class="prova">B0030-MRGENE1<br>(RBS-adhB)</td>
+                <td class="prova">Jul 28</td>
+		<td class="prova">1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a><br>sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B2-5</td>
+		<td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td>
+                <td class="prova">Jul 23</td>
+		<td class="prova">2</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B3-5</td>
+		<td class="prova">B1-B0015<br>(RBS-pdc-TT)</td>
+                <td class="prova">Jul 31</td>
+		<td class="prova">1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B4-2</td>
+		<td class="prova">B2-B0015<br>(RBS-adhB-TT)</td>
+                <td class="prova">Jul 31</td>
+		<td class="prova">1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">sequence ok (BMR)</td>
              </tr>
 </table>