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Team:Paris/Freezer PCR products
From 2009.igem.org
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|width=5%| A1 <span/ id="A1"> | |width=5%| A1 <span/ id="A1"> | ||
|width=10%| Tg3p_fusion N term | |width=10%| Tg3p_fusion N term | ||
| - | |width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers# | + | |width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O18 O18 ] |
| - | |width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers# | + | |width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ] |
|width=5%| ? (63.5) | |width=5%| ? (63.5) | ||
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] | |width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] | ||
|width=10%| 274 | |width=10%| 274 | ||
|width=25%| Amplification of N-term domain of M13's g3p | |width=25%| Amplification of N-term domain of M13's g3p | ||
| - | |width=5%| | + | |width=5%| ? |
|width=5%| ? | |width=5%| ? | ||
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05] | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05] | ||
Revision as of 12:30, 9 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
PCR products
| PCR products | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ref | Name | Oligo Fw | Oligo Rev | Tm used(Tm cal.) (°C) | Matrix | Length (bp) | Comments | Quantity | [ ] (ng/µL) | Date |
| A1 | Tg3p_fusion N term | O18 | O17 | ? (63.5) | F4 | 274 | Amplification of N-term domain of M13's g3p | ? | ? | 08/05 |
| A2 | ? | [O15] TE3_fusion_Fw | [O16] TE3_Rw | ? | F4 | 1036bp | Amplification of N-term domain of colicin E3 | ? | ? ng/µL | 08/05 |
| A3 | ? | [O31] clyA_RBS_Fw | [O10] clyA_fusion_polyG_Rw | ? | F4 | 987bp | Amplification of clyA with RBS in the primer | ? | ? ng/µL | 08/05 |
| A4 | ? | [O11] ompA_signal_Fw | [O12] ompA_signal_fusion_Rw | ? | F4 | 135bp | Amplification of OmpA signal for export to periplasm | ? | ? ng/µL | 08/05 |
| A5 | ? | [O1] Rev P1 | [O13] tolR_II_Fusion_Fw | ? | F4 | 279bp | Amplification of tolR | ? | ? ng/µL | 08/05 |
| A6 | ? | [O14] tatABC_RBS_Fw | [O5] Rev P5 | ? | F4 | 1634bp | Amplification of tatA with RBS | ? | ? ng/µL | 08/05 |
| A? | g3p_std | [O19] g3p_F | [O20] g3p_R | 78°C | P12 | 1287bp | For standardisation of g3p and adding SacI site | ? | ? ng/µL | ? |
| A? | OmpA_L_std | [O21] Ompa_linker_F | [O22] Ompa_linker_R | 80°C | P6 | 525bp | For standardisation of OmpA linker (BBaK103006) | ? | ? ng/µL | ? |
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
http://parts.mit.edu/images/partbypart/icon_regulatory.png
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
