Team:Paris/Protocols

From 2009.igem.org

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* Digestion
* Digestion
* Ligation
* Ligation
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====A.2. iGEM paris 2008 protocols link====
 
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[https://2008.igem.org/Team:Paris/Notebook/Protocols Click here]
 
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<u>content :</u>
 
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*Electrophoresis
 
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*Concentration of the Miniprep or the Midiprep
 
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*Amplification of promoters.
 
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*PCR Screening
 
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*<strike>Protocol to make competent bacteria</strike>
 
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====A.3. iGEM paris 2007 protocols link====
 
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[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
 
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<u>content :</u>
 
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*Growing_bacteria_in_liquid_medium
 
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*Preparing growth media
 
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**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
 
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*Chemical transformation
 
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*Glycerol Stock
 
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*Recombineering/Lambda red-mediated gene replacement
 
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*Miniprep
 
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*Fluorescent single cells visualisation
 
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*Digestion reactions
 

Revision as of 14:04, 10 August 2009

Contents

Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.

Main - Microscope Protocol - Adapted Protocols - Culture protocols - Molecular Biology
Main

Summary

1. Microscopy

  • Protocols suggested
  • [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]

2. Adapted Protocols

3. Culture protocols

4. Molecular biology

  • PCR
  • Gels
  • Digestion
  • Ligation

Retrieved from "http://2009.igem.org/Team:Paris/Protocols"