Revision as of 13:59, 11 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
2260	/	forward primer for		45µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
2260	/	forward primer for			100μM	aug 10
2261	/	reverse primer for			100μM	aug 10
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region	restricted with EcoRI			july 29
	/	BLR promoter region	4 microcentrifuge tubes			july 30
Template:IGempart	/	kanamycine pasmide backbone	taken from plates	13μl		july 29
Template:IGempart	/	constitutive promotor	4 microcentrifuge tubes	13μl		july 30

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5
pSB1A2	Sam8 + rbs	purified plasmid	95,7 ng/µl	August 5
pSB1A2	Sam8	purified plasmid	81,5 ng/µl	August 5
pSB1A2	COMT	purified plasmid	122,9 ng/µl	August 5
pSB1A2	COMT + rbs	plasmid from registry		August 6
pSB1A2	ech + rbs	purified digest	16,9 ng/µl	August 6
	fcs + rbs	purified digest	16,6 ng/µl	August 6
pSB1A2	sam5 + rbs	purified digest	3,1 ng/µl	August 7
	sam8 + rbs	purified digest	3,4 ng/µl	August 7

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	aug 10
	pSB1A2	medium constitutieve promoter			july 23
ampicilline

@@ Line 37: / Line 37: @@
 | {{kulpart|BBa_K238000}}||/||BLR promoter region||4 microcentrifuge tubes||||||july 30
 |-
+| {{iGempart|BBa_pSB3K3-P1010}}||/||kanamycine pasmide backbone|| taken from plates || 13μl ||||july 29
+|-
+| {{iGempart|BBa_J23101}}||/||constitutive promotor||4 microcentrifuge tubes|| 13μl ||||july 30
 |}