Revision as of 12:05, 12 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
2260	/	forward primer for		45µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
2260	/	forward primer for			100μM	aug 10
2261	/	reverse primer for			100μM	aug 10
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region (1:200 adn 1:500)	restricted with EcoRI			july 29
	/	PCR BLR promoter region (360bp)	4 microcentrifuge tubes			july 30
Template:IGempart	/	kanamycine pasmide backbone	taken from plates	13μl		aug 11
Template:IGempart	/	constitutive promotor	taken from plates	13μl		aug 11
Template:IGempart	/	Exact blue light promotor	cut with EcoRI and SpeI	26μl		aug 12

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

plasmid	description	status	μl left	concentration	date
pSB1A2	ech + rbs	purified plasmid (C2)		108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid (C1)		113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh			August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid (A2)		101,3 ng/µl	August 5
pSB1A2	Sam8 + rbs	purified plasmid (A1)		95,7 ng/µl	August 5
pSB1A2	Sam8	purified plasmid		81,5 ng/µl	August 5
pSB1A2	COMT	purified plasmid (B2)		122,9 ng/µl	August 5
pSB1A2	COMT + rbs	plasmid from registry			August 6
pSB1A2	ech + rbs	purified digest		16,9 ng/µl	August 6
	fcs + rbs	purified digest		16,6 ng/µl	August 6
pSB1A2	sam5 + rbs	purified digest		3,1 ng/µl	August 7
	sam8 + rbs	purified digest		3,4 ng/µl	August 7
pSB1A2	fcs + rbs	purified plasmid (fcs pl.)	41 µl	57,2 ng/µl	August 12
pSB1A2	Sam5 + rbs	purified plasmid (sam5 pl.)	43 µl	76,0 ng/µl	August 12
pSB1A2	Sam8 + rbs	purified plasmid (sam8 pl.)	17 µl	45,2 ng/µl	August 12

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	aug 10
	pSB1A2	medium constitutieve promoter			july 23
ampicilline

@@ Line 70: / Line 70: @@
 ! class='top' | date
 |-
-|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid||||108,8 ng/µl||August 4
+|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (C2)||||108,8 ng/µl||August 4
 |-
-|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid||||113,9 ng/µl||August 4
+|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (C1)||||113,9 ng/µl||August 4
 |-
 |{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
 |-
-|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid||||101,3 ng/µl||August 5
+|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (A2)||||101,3 ng/µl||August 5
 |-
-|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid||||95,7 ng/µl||August 5
+|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (A1)||||95,7 ng/µl||August 5
 |-
 |{{kulpart|BBa_I742142}}||pSB1A2||Sam8||purified plasmid||||81,5 ng/µl||August 5
 |-
-|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid||||122,9 ng/µl||August 5
+|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (B2)||||122,9 ng/µl||August 5
 |-
 |{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||||||August 6