Team:KULeuven/Freezer
From 2009.igem.org
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|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (B2)||||122,9 ng/µl||August 5 | |{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (B2)||||122,9 ng/µl||August 5 | ||
|- | |- | ||
- | |{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||||||August 6 | + | |{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||0 µl||||August 6 |
|- | |- | ||
|||pSB1A2||ech + rbs||purified digest||||16,9 ng/µl||August 6 | |||pSB1A2||ech + rbs||purified digest||||16,9 ng/µl||August 6 |
Revision as of 12:09, 12 August 2009
Contents |
Blue light receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
MC4100 | / | strain of E.coli | july 24 | |||
2171 | / | forward primer for | 90µl | 20μM | july 24 | |
2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
2171 | / | forward primer for | 100μM | july 24 | ||
2172 | / | reverse primer for | 100μM | july 24 | ||
2260 | / | forward primer for | 45µl | 20μM | aug 10 | |
2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
2260 | / | forward primer for | 100μM | aug 10 | ||
2261 | / | reverse primer for | 100μM | aug 10 | ||
pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
/ | BLR promoter region (1:200 adn 1:500) | restricted with EcoRI | july 29 | |||
/ | PCR BLR promoter region (360bp) | 4 microcentrifuge tubes | july 30 | |||
Template:IGempart | / | kanamycine pasmide backbone | taken from plates | 13μl | aug 11 | |
Template:IGempart | / | constitutive promotor | taken from plates | 13μl | aug 11 | |
Template:IGempart | / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | aug 12 |
Key/antikey/lock
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
/ | GFP with LVA tag | purified | july 28 | |||
/ | tetR promoter | purified | july 28 |
vanillin synthesis
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
pSB1A2 | ech + rbs | purified plasmid (C2) | 108,8 ng/µl | August 4 | ||
pSB1A2 | fcs + rbs | purified plasmid (C1) | 113,9 ng/µl | August 4 | ||
pSB1A2 | Sam5 + rbs | received from Edinburgh | August 3 | |||
pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid (A2) | 101,3 ng/µl | August 5 | ||
pSB1A2 | Sam8 + rbs | purified plasmid (A1) | 95,7 ng/µl | August 5 | ||
pSB1A2 | Sam8 (b) | purified plasmid | 81,5 ng/µl | August 5 | ||
pSB1A2 | COMT | purified plasmid (B2) | 122,9 ng/µl | August 5 | ||
pSB1A2 | COMT + rbs | plasmid from registry | 0 µl | August 6 | ||
pSB1A2 | ech + rbs | purified digest | 16,9 ng/µl | August 6 | ||
fcs + rbs | purified digest | 16,6 ng/µl | August 6 | |||
pSB1A2 | sam5 + rbs | purified digest | 3,1 ng/µl | August 7 | ||
sam8 + rbs | purified digest | 3,4 ng/µl | August 7 | |||
pSB1A2 | fcs + rbs | purified plasmid (fcs pl.) | 41 µl | 57,2 ng/µl | August 12 | |
pSB1A2 | Sam5 + rbs | purified plasmid (sam5 pl.) | 43 µl | 76,0 ng/µl | August 12 | |
pSB1A2 | Sam8 + rbs | purified plasmid (sam8 pl.) | 17 µl | 45,2 ng/µl | August 12 |
vanillin receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
pSB1A2 | ech + rbs | purified plasmid | 108,8 ng/µl | August 4 | ||
pSB1A2 | fcs + rbs | purified plasmid | 113,9 ng/µl | August 4 | ||
pSB1A2 | Sam5 + rbs | received from Edinburgh | August 3 | |||
pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid | 101,3 ng/µl | August 5 |
miscellaneous
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
pSB1A2 | medium constitutieve promoter | july 23 | ||||
ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238000 | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742105 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa I742142 | Team:KULeuven/BioBricks/BBa I742107 | Team:KULeuven/BioBricks/BBa I742109 | Team:KULeuven/BioBricks/J23110