Revision as of 15:11, 13 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
2260	/	forward primer for		45µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
2260	/	forward primer for			100μM	aug 10
2261	/	reverse primer for			100μM	aug 10
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region (1:200 adn 1:500)	restricted with EcoRI			july 29
	/	PCR BLR promoter region (360bp)	4 microcentrifuge tubes			july 30
Template:IGempart	pSB3K3	kanamycine plasmide backbone	taken from plates	13μl		aug 11
Template:IGempart	/	constitutive promotor	taken from plates	13μl		aug 11
Template:IGempart	/	Exact blue light promotor	cut with EcoRI and SpeI	26μl	27 ng/μl /	aug 12

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

plasmid	description	status	μl left	concentration	date
pSB1A2	ech + rbs	purified plasmid (oranje)		108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid (oranje)		113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh			August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid (oranje)		101,3 ng/µl	August 5
pSB1A2	Sam8 + rbs	purified plasmid (oranje)		95,7 ng/µl	August 5
pSB1A2	Sam8 (b)	purified plasmid (oranje)		81,5 ng/µl	August 5
pSB1A2	COMT	purified plasmid (oranje)		122,9 ng/µl	August 5
pSB1A2	COMT + rbs	plasmid from registry	0 µl		August 6
pSB1A2	ech + rbs	purified digest (C2)		16,9 ng/µl	August 6
	fcs + rbs	purified digest (C1)		16,6 ng/µl	August 6
pSB1A2	sam5 + rbs	purified digest (A2)		3,1 ng/µl	August 7
	sam8 + rbs	purified digest (A1)		3,4 ng/µl	August 7
pSB1A2	sam5 + sam8	ligation (lig. A)			August 11
pSB1A2	ech + fcs	ligation (lig.C)			August 11
pSB1A2	fcs + rbs	purified plasmid (fcs pl.)	39 µl	57,2 ng/µl	August 12
pSB1A2	Sam5 + rbs	purified plasmid (sam5 pl.)	41 µl	76,0 ng/µl	August 12
pSB1A2	Sam8 + rbs	purified plasmid (sam8 pl.)	15 µl	45,2 ng/µl	August 12
	fcs + rbs	purified digest (F rd.)	28 µl	4,2 ng/µl	August 12
pSB1A2	sam5 + rbs	purified digest (5 rd.)	28 µl	7,3 ng/µl	August 12
pSB1A2	ech + rbs	purified plasmid (ech pl.)	48 µl	72,7 ng/µl	August 13
pSB1A2	Sam8 + rbs	purified plasmid (sam8 pl.)	48 µl	66,2 ng/µl	August 13
pSB1A2	sam5 + sam8	cells with sam5+sam8 (elek. A)	600 µl		August 13
pSB1A2	ech + fcs	cells with ech+fcs (elek. C)	600 µl		August 13

plasmid	description	concentration	date
/	forward primer for	20μM	August 3
/	reverse primer for	20μM	August 3
/	forward primer for	100μM	August 3
/	reverse primer for	100μM	August 3
/	forward primer for	20μM	August 3
/	reverse primer for	20μM	August 3
/	forward primer for	100μM	August 3
/	reverse primer for	100μM	August 3
/	forward primer for	20μM	August 3
/	reverse primer for	20μM	August 3
/	forward primer for	100μM	August 3
/	reverse primer for	100μM	August 3
/	forward primer for	20μM	August 3
/	reverse primer for	20μM	August 3
/	forward primer for	100μM	August 3
/	reverse primer for	100μM	August 3

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	aug 10
	pSB1A2	medium constitutieve promoter			july 23
ampicilline

@@ Line 93: / Line 93: @@
 |-
 |||||sam8 + rbs||purified digest (A1)||||3,4 ng/µl ||August 7
+|-
+|||pSB1A2||sam5 + sam8||ligation (lig. A)||||||August 11
+|-
+|||pSB1A2||ech + fcs||ligation (lig.C)||||||August 11
 |-
 |{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs pl.)||39 µl||57,2 ng/µl||August 12
@@ Line 103: / Line 107: @@
 |-
 |||pSB1A2||sam5 + rbs||purified digest (5 rd.)||28 µl||7,3 ng/µl||August 12
-|-
-|||pSB1A2||sam5 + sam8||ligation (lig. A)||||||August 12
-|-
-|||pSB1A2||ech + fcs||ligation (lig.C)||||||August 12
 |-
 |{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech pl.)||48 µl||72,7 ng/µl||August 13