Template:Team:KULeuven/13 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) (New page: # PCR product of 12-august of pairt {{kulpart|BBa_K238013}} # Miniprep of {{kulpart|BBa_J23101}} Nanodrop results: {| border ="1" align="center" !| Part || concentration (ng/μl) || 260...) |
JochemDeen (Talk | contribs) |
||
Line 5: | Line 5: | ||
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | !| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | ||
|- align="center" | |- align="center" | ||
- | | {{kulpart|BBa_K238013}} ||123 || 1,82 || | + | | {{kulpart|BBa_K238013}} ||123,3 || 1,82 || |
|- align="center" | |- align="center" | ||
- | | {{kulpart|BBa_J23101}} ||141|| 1,99 || | + | | {{kulpart|BBa_J23101}} ||141,2|| 1,99 || |
|- | |- | ||
|} | |} |
Revision as of 15:39, 13 August 2009
- PCR product of 12-august of pairt
- Miniprep of
Nanodrop results:
Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
---|---|---|---|
123,3 | 1,82 | ||
141,2 | 1,99 |
- Restriction digest cut with EcoRI and XbaI
- Electroporation of Psb3k3 + P1010 and the ligation of ...
- Gelelectrophorese of
- Only 1 band, so probably everything was cut
- Gelectraction of
- Nanodrop = 29,3 ng/μl
- ligation of vector and insert