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Template:Team:KULeuven/13 August 2009/VanillinProduction
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# Miniprep of Ech and Sam8 | # Miniprep of Ech and Sam8 | ||
#* Nanodrop results: | #* Nanodrop results: | ||
| + | #: | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | !| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | ||
| Line 13: | Line 14: | ||
|- | |- | ||
|} | |} | ||
| - | + | # Restriction digest on Ech and Sam8 | |
| - | + | # Gelelectrophorese on Ech and Sam8 | |
| - | + | # New electrocompetent cells were used for the electroporation using the old ligations | |
| - | + | ||
#* Lig A -> Sam5 + Sam8 | #* Lig A -> Sam5 + Sam8 | ||
#* Lig C -> Ech + fcs | #* Lig C -> Ech + fcs | ||
Revision as of 12:42, 14 August 2009
- Plated ComT (+RBS) from the registry
- Growing overnight in 37C
- Plated Ech
- From newly made liquid culture
- Miniprep of Ech and Sam8
- Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| Ech | 72,7 | 1,94 | |
| Sam8 | 66,2 | 2,00 |
- Restriction digest on Ech and Sam8
- Gelelectrophorese on Ech and Sam8
- New electrocompetent cells were used for the electroporation using the old ligations
- Lig A -> Sam5 + Sam8
- Lig C -> Ech + fcs
- Note: there was no spark
- Plating of the electroporated cells
- 200 μl per plate twice
- 600 μl left of A and C
