Template:Team:KULeuven/13 August 2009/VanillinProduction

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# Miniprep of Ech and Sam8
# Miniprep of Ech and Sam8
#* Nanodrop results:
#* Nanodrop results:
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#: {| border ="1" align="center" !| Part || concentration (ng/&mu;l) || 260/280 &lambda; || 260/230 &lambda; |- align="center" | Ech ||72,7 || 1,94 || |- align="center" | Sam8 ||66,2|| 2,00 ||   |- |}
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  {| border ="1" align="center"
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!| Part || concentration (ng/&mu;l) || 260/280 &lambda; || 260/230 &lambda;
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|- align="center"
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| Ech ||72,7 || 1,94 ||  
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|- align="center"
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| Sam8 ||66,2|| 2,00 ||
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|}
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# Restriction digest on Ech and Sam8
# Restriction digest on Ech and Sam8
# Gelelectrophorese on Ech and Sam8
# Gelelectrophorese on Ech and Sam8

Revision as of 12:50, 14 August 2009

  1. Plated ComT (+RBS) from the registry
    • Growing overnight in 37C
  2. Plated Ech
    • From newly made liquid culture
  3. Miniprep of Ech and Sam8
    • Nanodrop results:
    {| border ="1" align="center" !| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ |- align="center" | Ech ||72,7 || 1,94 || |- align="center" | Sam8 ||66,2|| 2,00 || |- |}
  4. Restriction digest on Ech and Sam8
  5. Gelelectrophorese on Ech and Sam8
  6. New electrocompetent cells were used for the electroporation using the old ligations
    • Lig A -> Sam5 + Sam8
    • Lig C -> Ech + fcs
    • Note: there was no spark
  7. Plating of the electroporated cells
    • 200 μl per plate twice
    • 600 μl left of A and C
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