Team:KULeuven/Freezer

From 2009.igem.org

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(→vanillin synthesis)
(→Blue light receptor)
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| {{iGempart|BBa_K238013}}||/||Exact blue light promotor ||cut with EcoRI and SpeI || 26μl || 27 ng/μl /||aug 12
| {{iGempart|BBa_K238013}}||/||Exact blue light promotor ||cut with EcoRI and SpeI || 26μl || 27 ng/μl /||aug 12
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|-
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| {{iGempart|BBa_K238013+ BBa_E0240}}||/||Exact blue light promotor ligated with E0240 in pSB1A2 ||T4 DNA ligation || 18μl || ||aug 13
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|-
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| {{iGempart|BBa_K238013+ BBa_K145015}}||/||Exact blue light promotor ligated with K145015 in pSB1A2 ||T4 DNA ligation || 18μl || ||aug 14
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Revision as of 13:34, 14 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter region (1:200 adn 1:500)restricted with EcoRIjuly 29
/PCR BLR promoter region (360bp)4 microcentrifuge tubesjuly 30
Template:IGempart pSB3K3 kanamycine plasmide backbone taken from plates 13μl aug 11
Template:IGempart/constitutive promotortaken from plates 13μl aug 11
Template:IGempart/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl /aug 12
Template:IGempart/Exact blue light promotor ligated with E0240 in pSB1A2 T4 DNA ligation 18μl aug 13
Template:IGempart/Exact blue light promotor ligated with K145015 in pSB1A2 T4 DNA ligation 18μl aug 14

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (oranje)108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid (oranje)113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (oranje)101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid (oranje)95,7 ng/µlAugust 5
pSB1A2Sam8 (b)purified plasmid (oranje)81,5 ng/µlAugust 5
pSB1A2COMTpurified plasmid (oranje)122,9 ng/µlAugust 5
pSB1A2COMT + rbsplasmid from registry0 µlAugust 6
pSB1A2ech + rbspurified digest (C2)16,9 ng/µlAugust 6
fcs + rbspurified digest (C1, FCS Rb)16,6 ng/µlAugust 6
pSB1A2sam5 + rbspurified digest (A2)3,1 ng/µlAugust 7
sam8 + rbspurified digest (A1)3,4 ng/µl August 7
pSB1A2sam5 + sam8ligation (lig. A)August 11
pSB1A2ech + fcsligation (lig.C)August 11
pSB1A2fcs + rbspurified plasmid (fcs pl.)39 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)41 µl76,0 ng/µlAugust 12
pSB1A2Sam8 + rbspurified plasmid (sam8 pl.)0 µl45,2 ng/µlAugust 12
fcs + rbspurified digest (F rd.)28 µl4,2 ng/µlAugust 12
pSB1A2sam5 + rbspurified digest (5 rd.)28 µl7,3 ng/µlAugust 12
pSB1A2ech + rbspurified plasmid (ech pl.)48 µl72,7 ng/µlAugust 13
pSB1A2Sam8 + rbspurified plasmid (sam8 pl.)48 µl66,2 ng/µlAugust 13
pSB1A2sam5 + sam8cells with sam5+sam8 (elek. A)600 µlAugust 13
pSB1A2ech + fcscells with ech+fcs (elek. C)600 µlAugust 13
sam8 + rbspurified digest (sam8 rb.)26 µl9,4 ng/µlAugust 14
pSB1A2ech + rbspurified digest (ech rb.)26 µl13,6 ng/µlAugust 14
pSB1A2sam8 + Sam5ligation (SAMS)30 µl August 14
pSB1A2ech + fcsligation (EF)30 µl August 14

vanillin receptor

part plasmid description status μl left concentration date
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline
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