Team:EPF-Lausanne/Last News
From 2009.igem.org
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Revision as of 14:58, 14 August 2009
Contents |
Keep track with what we did so far
(15.08.09)
- Protocol of ligation was refined.
- !!! Inducible LOVTAP and Read out biobrick were created.!!! The system is completed.
- They need to be sequenced, characterized and submitted to the registery.
(08.08.09)
- This fifth week of wetlab we have done the following things
- Problems in ligations.
(01.08.09)
- This fourth week of wetlab we have done the following things
- Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
- The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
- Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.
(24.07.09)
- This third week of wetlab we have done the following things
- Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
- Modeling part: Preliminar simulation was launched over the weekend.
(17.07.09)
- This second week of wetlab we have done the following things
- Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
- Modeling part: Files required to launch simulations were created and analyzed.
(12.07.09)
- This first week of wetlab we have done the following things
- Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
- Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
- Ordered and received the primers needed for the PCR of LovTAP
- Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
- Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
- Fused the two BioBricks "LacI" and "RBS"
- Digested the LovTAP PCR products and RBS part