Team:EPF-Lausanne/Last News

From 2009.igem.org

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:This fifth week of wetlab we have done the following things
*Problems in ligations.
*Problems in ligations.

Revision as of 14:58, 14 August 2009

Contents

                               


Last News




Keep track with what we did so far

(15.08.09)

  • Protocol of ligation was refined.
  • !!! Inducible LOVTAP and Read out biobrick were created.!!! The system is completed.
  • They need to be sequenced, characterized and submitted to the registery.

(08.08.09)

This fifth week of wetlab we have done the following things
  • Problems in ligations.

(01.08.09)

This fourth week of wetlab we have done the following things
  • Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
  • The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
  • Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.

(24.07.09)

This third week of wetlab we have done the following things
  • Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
  • Modeling part: Preliminar simulation was launched over the weekend.


(17.07.09)

This second week of wetlab we have done the following things
  • Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
  • Modeling part: Files required to launch simulations were created and analyzed.


(12.07.09)

This first week of wetlab we have done the following things
  • Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
  • Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
  • Ordered and received the primers needed for the PCR of LovTAP
  • Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
  • Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
  • Fused the two BioBricks "LacI" and "RBS"
  • Digested the LovTAP PCR products and RBS part