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August/16 August 2009
From 2009.igem.org
(Difference between revisions)
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[[Image:Planning_osaka.PNG]]<br> | [[Image:Planning_osaka.PNG]]<br> | ||
| - | 1.3O6HSL | + | ==1.3O6HSL P(tetR)+luxI<br>== |
| - | P(tetR)+luxI<br> | + | |
| + | 1.1.RBS + luxI<br> | ||
| + | <table border="1" Frame="box"> | ||
| + | <tr><td>Vector</td><td>Insert</td></tr> | ||
| + | <tr><td>RBS</td><td>luxI</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td><tr> | ||
| + | <tr><td>pSB1A2</td><td> </td></tr> | ||
| + | </table> | ||
| - | |||
| - | |||
| + | 1.2.RBS+ luxI + terminator<br> | ||
| + | <table border="1" Frame="box"> | ||
| + | <tr><td>Vector</td><td>Insert</td></tr> | ||
| + | <tr><td>double terminator</td><td>RBS+luxI</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td> </td></tr> | ||
| + | <tr><td>pSB1AK3</td><td></td></tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | 1.3.P(tetR)+RBS+ luxI + terminator<br> | ||
| + | <table border="1" Frame="box"> | ||
| + | <tr><td>Vector</td><td>Insert</td></tr> | ||
| + | <tr><td>BBa_R0040</td><td>RBS+luxI+terminator</td></tr> | ||
| + | <tr><td>pSB1A2</td><td> </td></tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | ==2.C4HSL P(tetR)+rhlI<br>== | ||
| + | |||
| + | 2.1.RBS + rhII | ||
| + | <table border="1" Frame="box"> | ||
| + | <tr><td>Vector</td><td>Insert</td></tr> | ||
| + | <tr><td>RBS</td><td>rhII</td></tr> | ||
| + | <tr><td>pSB1A2</td><td> </td></tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | 2.2.RBS + rhII + terminator | ||
| + | <table border="1" Frame="box"> | ||
| + | <tr><td>Vector</td><td>Insert</td></tr> | ||
| + | <tr><td>double terminator</td><td>RBS + rhII </td></tr> | ||
| + | <tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td> </td></tr> | ||
| + | <tr><td>pSB1AK3</td><td></td></tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | 2.3.P(tetR) + RBS + rhlI + terminator<br> | ||
| + | <table border="1" Frame="box"> | ||
| + | <tr><td>Vector</td><td>Insert</td></tr> | ||
| + | <tr><td>p(tetR)</td><td>RBS + rhII +terminator</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr> | ||
| + | <tr><td>pSB1A2</td><td></td></tr> | ||
| + | </table> | ||
Revision as of 08:52, 16 August 2009
parts planning
signal sensor
1.3O6HSL P(tetR)+luxI
1.1.RBS + luxI
| Vector | Insert |
| RBS | luxI |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | [http://partsregistry.org/Part:BBa_C0161 BBa_C0161] |
| pSB1A2 |
1.2.RBS+ luxI + terminator
| Vector | Insert |
| double terminator | RBS+luxI |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | |
| pSB1AK3 |
1.3.P(tetR)+RBS+ luxI + terminator
| Vector | Insert |
| BBa_R0040 | RBS+luxI+terminator |
| pSB1A2 |
2.C4HSL P(tetR)+rhlI
2.1.RBS + rhII
| Vector | Insert |
| RBS | rhII |
| pSB1A2 |
2.2.RBS + rhII + terminator
| Vector | Insert |
| double terminator | RBS + rhII |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | |
| pSB1AK3 |
2.3.P(tetR) + RBS + rhlI + terminator
| Vector | Insert |
| p(tetR) | RBS + rhII +terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| pSB1A2 |
3.AI-1
P(tetR)+lasI
4.3OH,C14:1-HSL
P(tetR)+cinI
signal receiver
1.3O6HSL
luxpL+luxR+luxpR
2.C4HSL
p(tetR) + rhlR + RhlR+C4
3.AI-1
p(tetR) + lasR + LasR+pAI
4.3OH,C14:1-HSL
p(tetR) + cinR + cinR
