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Team:SDU-Denmark/Protocols/Primer-PCR
From 2009.igem.org
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[[Team:SDU-Denmark|Home]] | [[Team:SDU-Denmark/Background|Background]] | [[Team:SDU-Denmark/Project|Project]] | [[Team:SDU-Denmark/Parts|Parts]] | [[Team:SDU-Denmark/Team|Team]] | [[Team:SDU-Denmark|Home]] | [[Team:SDU-Denmark/Background|Background]] | [[Team:SDU-Denmark/Project|Project]] | [[Team:SDU-Denmark/Parts|Parts]] | [[Team:SDU-Denmark/Team|Team]] | ||
[[Team:SDU-Denmark/Diary|Diary]] | [[Team:SDU-Denmark/Protocols|Protocols]] | [[Team:SDU-Denmark/Downloads|Downloads]] | [[Team:SDU-Denmark/Brainstorm|Brainstorm]] | [[Team:SDU-Denmark/Diary|Diary]] | [[Team:SDU-Denmark/Protocols|Protocols]] | [[Team:SDU-Denmark/Downloads|Downloads]] | [[Team:SDU-Denmark/Brainstorm|Brainstorm]] | ||
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=Protocol for Primer PCR= | =Protocol for Primer PCR= | ||
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| 7. || Hold || 4˚C || | | 7. || Hold || 4˚C || | ||
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Revision as of 10:04, 17 August 2009
Home | Background | Project | Parts | Team
Diary | Protocols | Downloads | Brainstorm
Contents |
Protocol for Primer PCR
dNTP mix (10mM)
10ul 100mM dGTP
10ul 100mM dATP
10ul 100mM dTTP
10ul 100mM dCTP
60ul H2O
(Total V=100ul)
PCR reaction
5.0ul Pfx-buffer (÷MgSO4)
1.0ul 50mM MgSO4
1.5ul 10mM dNTP mix
1.5ul forward primer
1.5ul reverse primer
39ul H2O
0.5ul Pfx enzyme (add just before PCR run)
PCR program
| 1. | Start | 94˚C | 2min |
| 2. | Denaturing | 94˚C | 1min |
| 3. | Annealing | 52˚C | 30sek |
| 4. | Elongering | 70˚C | 2min |
| 5. | GOTO 2 | rep. 29x | |
| 6. | Slut | 70˚C | 5min |
| 7. | Hold | 4˚C |
[http://www.example.com link title]
