"
Template:Team:KULeuven/18 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
* We propose 3 tests to check if the restriction digest (esp. between EcoRI and XbaI, because they lie very close together) works properly: | * We propose 3 tests to check if the restriction digest (esp. between EcoRI and XbaI, because they lie very close together) works properly: | ||
| - | + | #* Cut every plasmid (Sam8, Sam5, Ech, Fcs) separately with EcoRI, XbaI and -only Sam8 and Fcs- with SpeI. | |
| - | + | #* Cut subsequently with EcoRI and XbaI, leaving an incubation period of 1h between two digests. Total: 30µl | |
| - | + | #* Use pUC18 vector | |
* Made fluid cultures of Sam8, Sam5, Ech, Fcs | * Made fluid cultures of Sam8, Sam5, Ech, Fcs | ||
Revision as of 09:05, 20 August 2009
- Cells didn't grow. Yet again...
- Re-plated the ligation products (SAMS and EF)
- We propose 3 tests to check if the restriction digest (esp. between EcoRI and XbaI, because they lie very close together) works properly:
- Cut every plasmid (Sam8, Sam5, Ech, Fcs) separately with EcoRI, XbaI and -only Sam8 and Fcs- with SpeI.
- Cut subsequently with EcoRI and XbaI, leaving an incubation period of 1h between two digests. Total: 30µl
- Use pUC18 vector
- Made fluid cultures of Sam8, Sam5, Ech, Fcs
