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Team:KULeuven/Freezer
From 2009.igem.org
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|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (oranje)||||113,9 ng/µl||August 4 | |{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (oranje)||||113,9 ng/µl||August 4 | ||
|- | |- | ||
| - | |{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3 | + | |{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||Lost in transit||||August 3 |
|- | |- | ||
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (oranje)||||101,3 ng/µl||August 5 | |{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (oranje)||||101,3 ng/µl||August 5 | ||
Revision as of 14:15, 19 August 2009
Contents |
Blue light receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| MC4100 | / | strain of E.coli | july 24 | |||
| 2171 | / | forward primer for | 90µl | 20μM | july 24 | |
| 2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
| 2171 | / | forward primer for | 100μM | july 24 | ||
| 2172 | / | reverse primer for | 100μM | july 24 | ||
| 2260 | / | forward primer for | 45µl | 20μM | aug 10 | |
| 2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
| 2260 | / | forward primer for | 100μM | aug 10 | ||
| 2261 | / | reverse primer for | 100μM | aug 10 | ||
| pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
| pSB1A2 | GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | july 29 | |||
| / | BLR promoter region (1:200 adn 1:500) | restricted with EcoRI | july 29 | |||
| / | PCR BLR promoter region (360bp) | 4 microcentrifuge tubes | july 30 | |||
| pSB3K3 | kanamycine plasmide backbone | taken from plates | 13μl | aug 11 | ||
| / | Exact blue light promotor | pcr product | / | 123,3 ng/μl | aug 13 | |
| / | constitutive promotor | taken from plates | 13μl | aug 11 | ||
| / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12 | |
| / | LigA: Exact blue light promotor ligated with E0240 in pSB1A2 | T4 DNA ligation | 18μl | aug 13 | ||
| / | Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 | T4 DNA ligation | 18μl | aug 14 | ||
| / | Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9) | cut with EcoRI and PstI | 5 x 18μl | aug 18 |
Key/antikey/lock
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | GFP with LVA tag | purified | july 28 | |||
| / | tetR promoter | purified | july 28 |
vanillin synthesis
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| pSB1A2 | ech + rbs | purified plasmid (oranje) | 108,8 ng/µl | August 4 | ||
| pSB1A2 | fcs + rbs | purified plasmid (oranje) | 113,9 ng/µl | August 4 | ||
| pSB1A2 | Sam5 + rbs | received from Edinburgh | Lost in transit | August 3 | ||
| pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid (oranje) | 101,3 ng/µl | August 5 | ||
| pSB1A2 | Sam8 + rbs | purified plasmid (oranje) | 95,7 ng/µl | August 5 | ||
| pSB1A2 | Sam8 (b) | purified plasmid (oranje) | 81,5 ng/µl | August 5 | ||
| pSB1A2 | COMT | purified plasmid (oranje) | 122,9 ng/µl | August 5 | ||
| pSB1A2 | ech + rbs | purified digest (C2) | 16,9 ng/µl | August 6 | ||
| fcs + rbs | purified digest (C1, FCS Rb) | 16,6 ng/µl | August 6 | |||
| pSB1A2 | sam5 + rbs | purified digest (A2) | 3,1 ng/µl | August 7 | ||
| sam8 + rbs | purified digest (A1) | 3,4 ng/µl | August 7 | |||
| pSB1A2 | sam5 + sam8 | ligation (lig. A) | August 11 | |||
| pSB1A2 | ech + fcs | ligation (lig.C) | August 11 | |||
| pSB1A2 | fcs + rbs | purified plasmid (fcs pl.) | 29 µl | 57,2 ng/µl | August 12 | |
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 pl.) | 31 µl | 76,0 ng/µl | August 12 | |
| fcs + rbs | purified digest (F rd.) | 18 µl | 4,2 ng/µl | August 12 | ||
| pSB1A2 | sam5 + rbs | purified digest (5 rd.) | 18 µl | 7,3 ng/µl | August 12 | |
| pSB1A2 | ech + rbs | purified plasmid (ech pl.) | 38 µl | 72,7 ng/µl | August 13 | |
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 pl.) | 38 µl | 66,2 ng/µl | August 13 | |
| sam8 + rbs | purified digest (sam8 rd.) | 16 µl | 9,4 ng/µl | August 14 | ||
| pSB1A2 | ech + rbs | purified digest (ech rd.) | 16 µl | 13,6 ng/µl | August 14 | |
| pSB1A2 | sam8 + Sam5 | ligation (SAMS) | August 14 | |||
| pSB1A2 | ech + fcs | ligation (EF) | August 14 | |||
| pSB1A2 | ech + rbs | purified plasmid (ech A) | 36,8 µl | 88,6 ng/µl | August 19 | |
| pSB1A2 | ech + rbs | purified plasmid (ech B) | 48 µl | 96,7 ng/µl | August 19 | |
| pSB1A2 | ech + rbs | purified plasmid (ech C) | 48 µl | 105,6 ng/µl | August 19 | |
| pSB1A2 | fcs + rbs | purified plasmid (fcs A) | 30,3 µl | 84,8 ng/µl | August 19 | |
| pSB1A2 | fcs + rbs | purified plasmid (fcs B) | 48 µl | 113,4 ng/µl | August 19 | |
| pSB1A2 | fcs + rbs | purified plasmid (fcs C) | 48 µl | 90,3 ng/µl | August 19 | |
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 A) | 37,4 µl | 94,6 ng/µl | August 19 | |
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 B) | 48 µl | 125,0 ng/µl | August 19 | |
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 C) | 48 µl | 104,2 ng/µl | August 19 | |
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 A) | 21,9 µl | 58,2 ng/µl | August 19 | |
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 B) | 48 µl | 63,2 ng/µl | August 19 |
vanillin receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| 2235 | / | forward primer for | 20μM | August 3 | ||
| 2238 | / | reverse primer for | 20μM | August 3 | ||
| 2235 | / | forward primer for | 100μM | August 3 | ||
| 2238 | / | reverse primer for | 100μM | August 3 | ||
| 2242 | / | forward primer for | 20μM | August 3 | ||
| 2243 | / | reverse primer for | 20μM | August 3 | ||
| 2242 | / | forward primer for | 100μM | August 3 | ||
| 2243 | / | reverse primer for | 100μM | August 3 | ||
| 2244 | / | forward primer for | 20μM | August 3 | ||
| 2245 | / | reverse primer for | 20μM | August 3 | ||
| 2244 | / | forward primer for | 100μM | August 3 | ||
| 2245 | / | reverse primer for | 100μM | August 3 | ||
| 2246 | / | forward primer for | 20μM | August 3 | ||
| 2247 | / | reverse primer for | 20μM | August 3 | ||
| 2246 | / | forward primer for | 100μM | August 3 | ||
| 2247 | / | reverse primer for | 100μM | August 3 | ||
| 2288 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2289 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2288 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2289 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2290 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2291 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2290 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2291 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2292 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2293 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2292 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2293 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2294 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2295 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2294 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2295 | / | reverse primer for mutagenesis for | 100μM | August 8 |
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
| pSB1A2 | medium constitutieve promoter | july 23 | ||||
| ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238000 | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/BBa pSB3K3-P1010 | Team:KULeuven/BioBricks/BBa J23101 | Team:KULeuven/BioBricks/BBa K238013+ BBa E0240 | Team:KULeuven/BioBricks/BBa K238013+ BBa K145015 | Team:KULeuven/BioBricks/BBa K238013+ BBa E02040 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742105 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa I742142 | Team:KULeuven/BioBricks/BBa I742107 | Team:KULeuven/BioBricks/BBa K238008 | Team:KULeuven/BioBricks/BBa K238009 | Team:KULeuven/BioBricks/BBa K238010 | Team:KULeuven/BioBricks/BBa K238011 | Team:KULeuven/BioBricks/J23110
