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Template:Team:KULeuven/18 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
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| - | 1) miniprep of parts pSB3K3- | + | 1) miniprep of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and LigA BLP-{{kulpart|BBa_E0240}} |
2) Restriction digest of the DNA from step 1 with EcoRI and PstI | 2) Restriction digest of the DNA from step 1 with EcoRI and PstI | ||
3) gelelectrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesfull. | 3) gelelectrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesfull. | ||
Revision as of 11:51, 1 September 2009
1) miniprep of parts - and LigA BLP-
2) Restriction digest of the DNA from step 1 with EcoRI and PstI
3) gelelectrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesfull.
