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| | {{Template:Valencia09iGEM}} | | {{Template:Valencia09iGEM}} |
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| - | <div align="justify" style="position:relative; top:-5px; left:70px; width:500px">
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| - | == '''Yeast Team''' ==
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| - | <span style="color:black; align:justify; font-size:10pt; font-family: Verdana">
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| - | An important part of our project is to work with '''yeasts''' in order to create each and everyone of the cell-pixels of our bioscreen. We will use some aequorin-transformed yeast strains.
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| - | '''Aequorin''' is a luminiscent protein isolated from luminescent jellyfish and many other marine organisms that uses '''coelenterazine''' as its cofactor to produce light whenever Ca<SUP>2+</SUP> is bound to it. Using a '''chemical input''' (KOH, alkali shock) we will open '''Ca<SUP>2+</SUP> channels''' in the yeasts' membrane (Viladevall L, et al. J Biol Chem. (2004) 279 43614–43624), Ca<SUP>2+</SUP> will enter into the cells and we will get '''light''' as an '''output'''.
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| - | Later we will try to reproduce the same response with an '''electrical stimulus''', using some hardware built by ourselves.
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| - | [[Image:Wiki.JPG|400px|center]]
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| - | </span>
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| - | </div>
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| - | <html>
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| - | <head>
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| - | <script type="text/javascript">
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| - | function mouseOverCellTeam()
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| - | {
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| - | document.getElementById("b12").src="https://static.igem.org/mediawiki/2009/9/9a/CellTeam_off.gif"
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| - | }
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| - | function mouseOutCellTeam()
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| - | {
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| - | document.getElementById("b12").src="https://static.igem.org/mediawiki/2009/1/12/CellTeam_on.gif"
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| - | }
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| - | function mouseOverYeastTeam()
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| - | {
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| - | document.getElementById("b13").src="https://static.igem.org/mediawiki/2009/1/1d/YeastTeam_off.jpg"
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| - | }
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| - | function mouseOutYeastTeam()
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| - | {
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| - | document.getElementById("b13").src="https://static.igem.org/mediawiki/2009/a/a8/YeasTeam_on.jpg"
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| - | }
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| - | </script>
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| - | </head>
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| - | <body>
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| - | <div style="position:relative; left:690px; top: -520px">
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| - | <table border="0" cellpadding="0" cellspacing="0">
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| - | <tr>
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| - | <td><a href="https://2009.igem.org/Team:Valencia/CellTeam" target="_top" onmouseover="mouseOverCellTeam()"
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| - | onmouseout="mouseOutCellTeam()">
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| - | <img border="0" alt="Cell Team" src="https://static.igem.org/mediawiki/2009/1/12/CellTeam_on.gif" id="b12" /></a></td>
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| - | </tr>
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| - | <tr>
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| - | <td><a href="https://2009.igem.org/Team:Valencia/YeastTeam" target="_top" onmouseover="mouseOverYeastTeam()"
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| - | onmouseout="mouseOutYeastTeam()">
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| - | <img border="0" alt="Yeas Team" src="https://static.igem.org/mediawiki/2009/a/a8/YeasTeam_on.jpg" id="b13" /></a></td>
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| - | </tr>
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| - | </table>
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| - | </div>
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| - | </body>
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| - | </html>
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