August/21 August 2009

From 2009.igem.org

(Difference between revisions)
(check the result of transformation)
(restriction and digestion)
Line 16: Line 16:
===restriction and digestion===
===restriction and digestion===
-
ⅰ パーツ⑨の作成
+
ⅰ パーツ⑨
-
 Insert
+
Insert
-
  H2O       
+
H2O         4
-
 No.2 buffer 2
+
No.2 buffer  2
-
①ー3        12
+
①ー3       12
-
EcoRI
+
EcoRI       1
 +
SpeⅠ     

Revision as of 00:44, 22 August 2009

transformation

to get new plasmid

⑤(RBS+rhlR+terminator+promoter(reg))
⑦(RBS+cinR)
⑧(RBS+luxI)

check the result of transformation

2-8G     4
2-21F    0    
2-6O     2
2-13N   <15
⑤      0

restriction and digestion

ⅰ パーツ⑨ Insert H2O      4 No.2 buffer  2 ①ー3   12 EcoRI   1 SpeⅠ