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| | ==Coliguard: recovering the BioBricks== | | ==Coliguard: recovering the BioBricks== |
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| - | Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent E. coli (DH10B) according to protocol 3 (see protocol section). The cells were then plated in LB media containing the appropriate antibiotic.
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| - | '''Marcelo'''
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| - | Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss
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| - | Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030
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| - | '''Luige'''
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| - | Part:BBa_J61100 - Ribosome Binding Site Family Member
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| - | Part:BBa_J61107 - Ribosome Binding Site Family Member
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| - | '''Marcos'''
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| - | Part:BBa_B0010 - T1 from E. coli rrnB
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| - | Part:BBa_B0014 - double terminator
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| - | '''Leonardo'''
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| - | Part:BBa_B0015 - double terminator (B0010-B0012)
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| - | Part:BBa_R0040 - TetR repressible promoter
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| - | Part:BBa_J23100 - constitutive promoter family member
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| - | Part:BBa_J23107 - constitutive promoter family member
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| - | '''Fabiana'''
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| - | Part:BBa_J23109 - constitutive promoter family member
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| - | Part:BBa_J31007 - tetracycline resistance protein TetA(C)
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| - | Part:BBa_J31003 - kanamycin resistance forward
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| - | Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
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| - | '''Victor'''
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| - | Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata
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| - | Part:pSB1A3 - High copy BioBrick assembly plasmid
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| - | Part:pSB1AK3 - High copy BioBrick assembly plasmid
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| - | Part:pSB3K3 - Low to medium copy BioBrick standard vector
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| - | Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)
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| - | {{:Team:UNICAMP-Brazil/inc_rodape}}
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