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Template:Team:KULeuven/21 August 2009/VanillinProduction
From 2009.igem.org
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| - | * | + | * ''sam8'' and ''sam5''; ''fcs'' and ''ech'' were ligated |
Used volumes: | Used volumes: | ||
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!| Part || µl || | !| Part || µl || | ||
|- align="center" | |- align="center" | ||
| - | | | + | | ''sam8'' ||34 || |
|- align="center" | |- align="center" | ||
| - | | | + | | ''sam5'' ||6,3 || |
|- align="center" | |- align="center" | ||
| - | | | + | | ''ech'' ||10,0 || |
|- align="center" | |- align="center" | ||
| - | | | + | | ''fcs'' ||35,8 || |
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Revision as of 11:29, 14 September 2009
- Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The signals do not correspond with an EF-ligation product.
- The DNA was purified from the gel
Nanodrop results:
| Part | ng/µl | 260/280 | |
|---|---|---|---|
| Sam8 | 2,5 | 1,14 | |
| Sam5 | 7,9 | 1,60 | |
| Ech | 5,0 | 1,38 | |
| Fcs | 3,4 | 0,93 |
- sam8 and sam5; fcs and ech were ligated
Used volumes:
| Part | µl | |
|---|---|---|
| sam8 | 34 | |
| sam5 | 6,3 | |
| ech | 10,0 | |
| fcs | 35,8 |
