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Template:Team:KULeuven/11 August 2009/VanillinReceptor
From 2009.igem.org
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| + | * We also recieved another strain of Agrobacterium. We brought this new strain in culture. | ||
* From now on to make things clear the genes from the first Agrobacterium will be called A,B,G and R for respectively virA, promotor regin virB , virG and rpoA. For the second agrobacterium we will be using W,X,Y and Z as name for the same genes. | * From now on to make things clear the genes from the first Agrobacterium will be called A,B,G and R for respectively virA, promotor regin virB , virG and rpoA. For the second agrobacterium we will be using W,X,Y and Z as name for the same genes. | ||
* We achieved good results for genes B, G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampcillin. We isolated the good colonies again. | * We achieved good results for genes B, G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampcillin. We isolated the good colonies again. | ||
* There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A. | * There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A. | ||
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Revision as of 12:36, 25 August 2009
- We also recieved another strain of Agrobacterium. We brought this new strain in culture.
- From now on to make things clear the genes from the first Agrobacterium will be called A,B,G and R for respectively virA, promotor regin virB , virG and rpoA. For the second agrobacterium we will be using W,X,Y and Z as name for the same genes.
- We achieved good results for genes B, G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampcillin. We isolated the good colonies again.
- There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A.
