August/23 August 2009

From 2009.igem.org

(Difference between revisions)
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  (ligation sample No.) (no. of colonies)
  (ligation sample No.) (no. of colonies)
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+
          9                    1
-
+
          10                  100~
-
+
          11                  100~
-
+
-
+
   
   
  inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)
  inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)

Revision as of 03:51, 26 August 2009

1.transformed check Checked the cell cultures transformed and plated out yesterday (8/22) for colony formation and made an approximate count of the number of colonies.

(ligation sample No.) (no. of colonies)
          9                    1
         10                   100~
         11                   100~

inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)


2.digestion and ligation

'8/22 digestion sample

gel electrophoresis
gel cut

purification by [QIAquick Nucleotide Removal Kit]

ligation

ligation
DNA        44
10* buffer 5
ligation   1
total      50uL


16°C 3~4hr

transfromation


3.transfromation

ligation sample
⑤⑫⑬⑭⑮
2-18H
1-8E
1-4E
2-16N
2-16L