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Template:Team:KULeuven/26 August 2009/VanillinProduction
From 2009.igem.org
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-We performed 2 simultaneous digests: one to verify the length of the product (Restriction2Test) using SAMS I and EF III and another to prepare for ligation (Restriction4Real) with promotor and terminator using SAMS III and EF II | -We performed 2 simultaneous digests: one to verify the length of the product (Restriction2Test) using SAMS I and EF III and another to prepare for ligation (Restriction4Real) with promotor and terminator using SAMS III and EF II | ||
| - | -We cut with the 2 enzymes separately, allowing a one hour incubation between | + | -We cut with the 2 enzymes separately, allowing a one hour incubation in between |
* Terminator was also digested | * Terminator was also digested | ||
Revision as of 09:14, 26 August 2009
- Miniprep of fluid cultures
Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| SamS I | 53,9 | 1,87 | |
| SamS II | 39,4 | 2,16 | |
| SamS III | 57,1 | 2,07 | |
| EF I | 76,8 | 1,94 | |
| EF II | 83,4 | 2,04 | |
| EF III | 61,5 | 2,01 |
- Restriction:
-We performed 2 simultaneous digests: one to verify the length of the product (Restriction2Test) using SAMS I and EF III and another to prepare for ligation (Restriction4Real) with promotor and terminator using SAMS III and EF II
-We cut with the 2 enzymes separately, allowing a one hour incubation in between
- Terminator was also digested
