Team:KULeuven/Wetlab

From 2009.igem.org

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The labwork is divided into four groups, each working on a specific aspect of ''Essencia coli''
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'''Blue Light Receptor'''
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Purifying the promotor region and creating it into a new biobrick
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'''Vanillin Production'''
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Testing three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferrulic acid to Vanillin)and eventually combining the five enzymes.
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'''Vanillin Receptor'''
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Removing unwanted restriction sites, sequencing and combining all elements into a standard iGEM plasmid.
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'''Key/Lock/Antikey'''
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Ligating Key, Lock and Antikey into promotor plasmids and testing the system with a GFP assay.

Revision as of 09:55, 3 September 2009

The labwork is divided into four groups, each working on a specific aspect of Essencia coli


Blue Light Receptor

Purifying the promotor region and creating it into a new biobrick


Vanillin Production

Testing three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferrulic acid to Vanillin)and eventually combining the five enzymes.


Vanillin Receptor

Removing unwanted restriction sites, sequencing and combining all elements into a standard iGEM plasmid.


Key/Lock/Antikey

Ligating Key, Lock and Antikey into promotor plasmids and testing the system with a GFP assay.

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