Template:Team:KULeuven/12 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
Line 11: | Line 11: | ||
#* Annealing of 58C | #* Annealing of 58C | ||
# Innoculate liquid medium | # Innoculate liquid medium | ||
- | #* Innoculation of {{kulpart| | + | #* Innoculation of {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} |
# Ligation | # Ligation | ||
{| border ="1" align="center" | {| border ="1" align="center" |
Revision as of 12:03, 1 September 2009
- Gel electrophorese of BlP cut with EcoRI and SpeI
- Gel extraction of the Blp
Nanodrop results
Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
---|---|---|---|
Blp | 27 | 2,08 |
- PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
- Annealing of 58C
- Innoculate liquid medium
- Innoculation of - and
- Ligation
Vector | insert |
+ | (BLP) |
50ng -> 2,5 μl | 5 ng -> 0,3 μl |