Template:Team:KULeuven/12 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
Line 17: Line 17:
| Vector || insert  
| Vector || insert  
|- align="center"
|- align="center"
-
| {{kulpart|psB1A3}}+{{kulpart|BBa_P1010}} || {{kulpart|BBa_K238013}} (BLP)  
+
| {{kulpart|pSB1A3}}+{{kulpart|BBa_P1010}} || {{kulpart|BBa_K238013}} (BLP)  
|- align="center"
|- align="center"
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
|- align="center"
|- align="center"
|}
|}

Revision as of 12:09, 1 September 2009

  1. Gel electrophorese of BlP cut with EcoRI and SpeI
  2. Gel extraction of the Blp

Nanodrop results

Part concentration (ng/μl) 260/280 λ 260/230 λ
Blp 27 2,08
  1. PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
    • Annealing of 58C
  2. Innoculate liquid medium
    • Innoculation of - and
  3. Ligation
Vector insert
+ (BLP)
50ng -> 2,5 μl 5 ng -> 0,3 μl
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