Template:Team:KULeuven/11 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
 
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# Restriction digest
# Restriction digest
#* {{kulpart|BBa_K238013}} cut with EcoRI and SpeI, incubation for 1,5 hour
#* {{kulpart|BBa_K238013}} cut with EcoRI and SpeI, incubation for 1,5 hour
-
# Gel electrophorese of {{kulpart|BBa_K238013}}
+
# Gel electrophoresis of {{kulpart|BBa_K238013}}
-
#* The cut should be 109 bp
+
#* The cut piece should be 109 bp
# Restriction digest
# Restriction digest
# Electroporation of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} in electrocompetent cells
# Electroporation of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} in electrocompetent cells

Latest revision as of 08:42, 8 September 2009

  1. Restriction digest
    • cut with EcoRI and SpeI, incubation for 1,5 hour
  2. Gel electrophoresis of
    • The cut piece should be 109 bp
  3. Restriction digest
  4. Electroporation of parts - and in electrocompetent cells