Template:Team:KULeuven/11 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
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# Restriction digest | # Restriction digest | ||
#* {{kulpart|BBa_K238013}} cut with EcoRI and SpeI, incubation for 1,5 hour | #* {{kulpart|BBa_K238013}} cut with EcoRI and SpeI, incubation for 1,5 hour | ||
- | # Gel | + | # Gel electrophoresis of {{kulpart|BBa_K238013}} |
- | #* The cut should be 109 bp | + | #* The cut piece should be 109 bp |
# Restriction digest | # Restriction digest | ||
# Electroporation of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} in electrocompetent cells | # Electroporation of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} in electrocompetent cells |
Latest revision as of 08:42, 8 September 2009
- Restriction digest
- cut with EcoRI and SpeI, incubation for 1,5 hour
- Gel electrophoresis of
- The cut piece should be 109 bp
- Restriction digest
- Electroporation of parts - and in electrocompetent cells