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Template:Team:KULeuven/3 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
(New page: # a restriction digest on {{kulpart|BBa_J23101}} with EcoRI and SpeI was performed. this will cut the promotor out of its vector. this vector ({{kulpart|BBa_J61002}}) has a RFP reporter ge...) |
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| - | # a restriction digest on {{kulpart|BBa_J23101}} with EcoRI and SpeI was performed. this will cut the promotor out of its | + | # a restriction digest on {{kulpart|BBa_J23101}} with EcoRI and SpeI was performed. this will cut the promotor out of its vector ({{kulpart|BBa_J61002}}) which has a RFP reporter gene. we will put {{kulpart|BBa_k238013}} (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependend on our blue light promotor. we can then compare this with the intensity of RFP produced by {{kulpart|BBa_J23101}}. |
# glycerol stock was made from LigA and {{kulpart|BBa_J23101}}. they are stored in the -80°C in S&P F8 67-68 and 69-70. | # glycerol stock was made from LigA and {{kulpart|BBa_J23101}}. they are stored in the -80°C in S&P F8 67-68 and 69-70. | ||
| + | # Ecoli strain DB3.1 cells were made competent for electroporesis. | ||
| + | # gel electorforesis and extraction on {{kulpart|BBa_J23101}} | ||
Revision as of 15:09, 3 September 2009
- a restriction digest on with EcoRI and SpeI was performed. this will cut the promotor out of its vector () which has a RFP reporter gene. we will put (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependend on our blue light promotor. we can then compare this with the intensity of RFP produced by .
- glycerol stock was made from LigA and . they are stored in the -80°C in S&P F8 67-68 and 69-70.
- Ecoli strain DB3.1 cells were made competent for electroporesis.
- gel electorforesis and extraction on
