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Team:KULeuven/Freezer
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|{{kulpart|BBa_I742115}}||{{kulpart|pSB1A2}}||fcs + rbs||purified plasmid (fcs pl.)||29 µl||57,2 ng/µl||August 12 | |{{kulpart|BBa_I742115}}||{{kulpart|pSB1A2}}||fcs + rbs||purified plasmid (fcs pl.)||29 µl||57,2 ng/µl||August 12 | ||
Revision as of 07:19, 4 September 2009
Contents |
Blue light receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| MC4100 | / | strain of E.coli | july 24 | |||
| 2260 | / | forward primer for | 42,5µl | 20μM | aug 10 | |
| 2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
| GFP + rbs + terminator | Miniprepped | 100μl | 85,6ng/μl | july 29 | ||
| GFP + rbs + terminator | restricted with EcoRI and XbaI | 20μl | july 29 | |||
| GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | 30μl | 29,3 ng/μl | aug 14 | ||
| kanamycine plasmide backbone | taken from plates | 13μl | aug 11 | |||
| / | Exact blue light promotor | pcr product | 20μl | 123,3 ng/μl | aug 13 | |
| / | constitutive promotor | taken from plates | 13μl | aug 11 | ||
| / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12 | |
| + | LigA: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | aug 13 | ||
| + | Lig B: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | aug 14 | ||
| + | / | Lig A: Exact blue light promotor ligated with in (1,3,7,9) | cut with EcoRI and PstI | 4 x 18μl | aug 24 | |
| + | LigA: Exact blue light promotor ligated with in | Miniprep (4x) | 20μl | aug 18 | ||
| constitutive promotor | miniprep | 10μl | 141,2ng/μl | aug 14 | ||
| (A) | constitutive promotor | restriction digest with EcoRI en SpeI | 20μl | 20,8ng/μl | aug 24 | |
| (B) | constitutive promotor | restriction digest with EcoRI en SpeI | 10μl | 12,8ng/μl | aug 24 | |
| + | LigA (1): Exact blue light promotor ligated with in | Miniprep | 45μl | 110,6ng/μl | aug 31 | |
| + | LigA (2): Exact blue light promotor ligated with in | Miniprep | 43μl | 76,7ng/μl | aug 31 | |
| + | LigA (3): Exact blue light promotor ligated with in | Miniprep | 44μl | 84,8ng/μl | aug 31 | |
| + | LigA (4): Exact blue light promotor ligated with in | Miniprep | 34μl | 31,7ng/μl | aug 31 | |
| pSB1A2 | RBS + GFP + terminator | Miniprep | 45μl | 104,3ng/μl | aug 31 | |
| (A) | pSB1A2 | promotor | Miniprep | 48μl | 238,9ng/μl | sept 2 |
| (B) | pSB1A2 | promotor | Miniprep | 48μl | 248,8ng/μl | sept 2 |
Key/antikey/lock
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | GFP with LVA tag | purified | july 28 | |||
| / | tetR promoter | purified | july 28 |
vanillin synthesis
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| fcs + rbs | purified plasmid (fcs pl.) | 29 µl | 57,2 ng/µl | August 12 | ||
| Sam5 + rbs | purified plasmid (sam5 pl.) | 31 µl | 76,0 ng/µl | August 12 | ||
| ech + rbs | purified plasmid (ech B) | 42,8 µl | 96,7 ng/µl | August 19 | ||
| ech + rbs | purified plasmid (ech C) | 48 µl | 105,6 ng/µl | August 19 | ||
| fcs + rbs | purified plasmid (fcs B) | 43,6 µl | 113,4 ng/µl | August 19 | ||
| fcs + rbs | purified plasmid (fcs C) | 48 µl | 90,3 ng/µl | August 19 | ||
| Sam5 + rbs | purified plasmid (sam5 B) | 44 µl | 125,0 ng/µl | August 19 | ||
| Sam5 + rbs | purified plasmid (sam5 C) | 48 µl | 104,2 ng/µl | August 19 | ||
| Sam8 + rbs | purified plasmid (sam8 B) | 40,1 µl | 63,2 ng/µl | August 19 | ||
| sam8 + sam5 | ligation mix (SAMS) | August 24 | ||||
| fcs + ech | ligation mix (EF) | August 24 | ||||
| fcs + ech | purified ligation plasmid (EF I) | 48 µl | 53,9 ng/µl | August 4 | ||
| fcs + ech | purified ligation plasmid (EF II) | 42 µl | 39,4 ng/µl | August 4 | ||
| fcs + ech | purified ligation plasmid (EF III) | 39,9 µl | 57,1 ng/µl | August 4 | ||
| sam8 + sam5 | purified ligation plasmid (SAMS I) | 48 µl | 76,8 ng/µl | August 4 | ||
| sam8 + sam5 | purified ligation plasmid (SAMS II) | 35,3 | 83,4 ng/µl | August 4 | ||
| sam8 + sam5 | purified ligation plasmid (SAMS III) | 39,3 µl | 61,5 ng/µl | August 4 | ||
| sam8 + sam5 | purified ligation plasmid (SAMS II), not purified from gel | 5 µl | August 31 | |||
| sam8 + sam5 | purified ligation plasmid (SAMS I), purified from gel | 25 µl | August 31 | |||
| fcs + ech | purified ligation plasmid (EF II), purified from gel | 5 µl | August 31 | |||
| sam8 + sam5 | SAMS 1 (from 'mother plate') | 16 µl | 35,4 ng/µl | September 3 | ||
| sam8 + sam5 | SAMS 2 (from 'mother plate') | 19,9 µl | 49,3 ng/µl | September 3 | ||
| sam8 + sam5 | SAMS 3 (from 'mother plate') | 31,9 µl | 62,0 ng/µl | September 3 | ||
| sam8 + sam5 | SAMS 4 (from 'mother plate') | 33,6 µl | 78,3 ng/µl | September 3 | ||
| Terminator | Terminator, B0015 | ±20 µl | 181,4 | September 3 |
vanillin receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| 2235 | / | forward primer for | 20μM | August 3 | ||
| 2238 | / | reverse primer for | 20μM | August 3 | ||
| 2235 | / | forward primer for | 100μM | August 3 | ||
| 2238 | / | reverse primer for | 100μM | August 3 | ||
| 2242 | / | forward primer for | 20μM | August 3 | ||
| 2243 | / | reverse primer for | 20μM | August 3 | ||
| 2242 | / | forward primer for | 100μM | August 3 | ||
| 2243 | / | reverse primer for | 100μM | August 3 | ||
| 2244 | / | forward primer for | 20μM | August 3 | ||
| 2245 | / | reverse primer for | 20μM | August 3 | ||
| 2244 | / | forward primer for | 100μM | August 3 | ||
| 2245 | / | reverse primer for | 100μM | August 3 | ||
| 2246 | / | forward primer for | 20μM | August 3 | ||
| 2247 | / | reverse primer for | 20μM | August 3 | ||
| 2246 | / | forward primer for | 100μM | August 3 | ||
| 2247 | / | reverse primer for | 100μM | August 3 | ||
| 2288 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2289 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2288 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2289 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2290 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2291 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2290 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2291 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2292 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2293 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2292 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2293 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2294 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2295 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2294 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2295 | / | reverse primer for mutagenesis for | 100μM | August 8 |
miscellaneous
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
| medium constitutieve promoter | july 23 | |||||
| ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/pSB1A2 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/pSB3K3 | Team:KULeuven/BioBricks/BBa J23101 | Team:KULeuven/BioBricks/pSB1A3 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa B0015 | Team:KULeuven/BioBricks/BBa K238008 | Team:KULeuven/BioBricks/BBa K238009 | Team:KULeuven/BioBricks/BBa K238010 | Team:KULeuven/BioBricks/BBa K238011 | Team:KULeuven/BioBricks/BBa J23110
