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Team:KULeuven/11 August 2009
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Revision as of 08:41, 8 September 2009
Progress of parts
[edit] Blue Light Receptor
- Restriction digest
- cut with EcoRI and SpeI, incubation for 1,5 hour
- Gel electrophoresis of
- The cut piece should be 109 bp
- Restriction digest
- Electroporation of parts - and in electrocompetent cells
[edit] Vanillin Production
- There was no growth of sam8/sam5, fcs/ech and COMT plates.
- Possible reason is a problem with the electrocompetent cells
- Or the concentration of sam5/sam8 is too low
- Plan for tomorrow is to create new electrocompetent cells
- Prepared LB and glycerol today
- Made liquid cultures sam8, sam5, fcs and ech
- Made new agarplates with Ampicilin
[edit] Vanillin Receptor
- We also received another strain of Agrobacterium. We brought this new strain in culture.
- To make things clear the genes from the first Agrobacterium will from now on be called A,B,G and R for respectively virA, promotor region virB , virG and rpoA. For the second Agrobacterium we will use W,X,Y and Z to indicate the same genes.
- We achieved good results for genes B,G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampicillin. We isolated the good colonies again.
- There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heatshock all over for A.
