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Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
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== YeastGuard: RBS and CFP plasmid digestion == | == YeastGuard: RBS and CFP plasmid digestion == | ||
| - | - Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. | + | - Today we started the construction of our first biobrick. After quantificate the mini-prep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. |
- After the digestion we isolated the target bands from the agarose gel, according to the protocol X. | - After the digestion we isolated the target bands from the agarose gel, according to the protocol X. | ||
Revision as of 16:55, 5 September 2009
YeastGuard: RBS and CFP plasmid digestion
- Today we started the construction of our first biobrick. After quantificate the mini-prep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.
- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.
- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
Raíssa
