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Uppsala-Sweden/6 September 2009
From 2009.igem.org
Karl.brune (Talk | contribs) (→Picking Colonies) |
Karl.brune (Talk | contribs) |
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==Moving ON to fride== | ==Moving ON to fride== | ||
ON cultures for the lux system were moved to the iGEM fridge for MiniPrep tomorrow | ON cultures for the lux system were moved to the iGEM fridge for MiniPrep tomorrow | ||
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All plates show colonoies. | All plates show colonoies. | ||
After picking colonies -> fridge. | After picking colonies -> fridge. | ||
| - | --[[User:Karl.brune|Karl.brune]] 23: | + | --[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC) |
==Picking Colonies== | ==Picking Colonies== | ||
I picked colonies from plates into 10µl selective media. | I picked colonies from plates into 10µl selective media. | ||
| - | --[[User:Karl.brune|Karl.brune]] 23: | + | --[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC) |
5 Colonies of pirA (AC3) -> pirA-X | 5 Colonies of pirA (AC3) -> pirA-X | ||
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5 Colonies of b0034-pirA (AC3) -> RA-X | 5 Colonies of b0034-pirA (AC3) -> RA-X | ||
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5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X | 5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X | ||
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5 Colonies of B0015 (AC3) - T-X | 5 Colonies of B0015 (AC3) - T-X | ||
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5 Colonies of R0011-B0034 (AK3) -> RR-X | 5 Colonies of R0011-B0034 (AK3) -> RR-X | ||
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3 Colonies of E1A1-B0015 (AK3) -> E1A1X | 3 Colonies of E1A1-B0015 (AK3) -> E1A1X | ||
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3 Colonies of E1A2-B0015 (AK3) -> E1A2X | 3 Colonies of E1A2-B0015 (AK3) -> E1A2X | ||
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3 Colonies of E1A3-B0015 (AK3) -> E1A3X | 3 Colonies of E1A3-B0015 (AK3) -> E1A3X | ||
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3 Colonies of E1A4-B0015 (AK3) -> E1A4X | 3 Colonies of E1A4-B0015 (AK3) -> E1A4X | ||
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3 Colonies of E1B1-B0015 (AK3) -> E1B1X | 3 Colonies of E1B1-B0015 (AK3) -> E1B1X | ||
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3 Colonies of E1B2-B0015 (AK3) -> E1B2X | 3 Colonies of E1B2-B0015 (AK3) -> E1B2X | ||
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3 Colonies of E1B3-B0015 (AK3) -> E1B3X | 3 Colonies of E1B3-B0015 (AK3) -> E1B3X | ||
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3 Colonies of E1B4-B0015 (AK3) -> E1B4X | 3 Colonies of E1B4-B0015 (AK3) -> E1B4X | ||
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3 Colonies of E21-B0015 (AK3) -> E21X | 3 Colonies of E21-B0015 (AK3) -> E21X | ||
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3 Colonies of E22-B0015 (AK3) -> E22X | 3 Colonies of E22-B0015 (AK3) -> E22X | ||
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3 Colonies of E23-B0015 (AK3) -> E23X | 3 Colonies of E23-B0015 (AK3) -> E23X | ||
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3 Colonies of E24-B0015 (AK3) -> E24X | 3 Colonies of E24-B0015 (AK3) -> E24X | ||
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X= # of colony | X= # of colony | ||
| - | --[[User:Karl.brune|Karl.brune]] 23: | + | --[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC) |
==Colony PCR== | ==Colony PCR== | ||
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Colony tubes were moved to fridge after taking template for the PCR | Colony tubes were moved to fridge after taking template for the PCR | ||
| - | --[[User:Karl.brune|Karl.brune]] 23: | + | --[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC) |
Revision as of 23:09, 5 September 2009
Contents |
Moving ON to fride
ON cultures for the lux system were moved to the iGEM fridge for MiniPrep tomorrow
Checking Plates
All plates show colonoies. After picking colonies -> fridge. --Karl.brune 23:08, 5 September 2009 (UTC)
Picking Colonies
I picked colonies from plates into 10µl selective media. --Karl.brune 23:08, 5 September 2009 (UTC) 5 Colonies of pirA (AC3) -> pirA-X 5 Colonies of b0034-pirA (AC3) -> RA-X 5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X 5 Colonies of B0015 (AC3) - T-X 5 Colonies of R0011-B0034 (AK3) -> RR-X
3 Colonies of E1A1-B0015 (AK3) -> E1A1X 3 Colonies of E1A2-B0015 (AK3) -> E1A2X 3 Colonies of E1A3-B0015 (AK3) -> E1A3X 3 Colonies of E1A4-B0015 (AK3) -> E1A4X
3 Colonies of E1B1-B0015 (AK3) -> E1B1X 3 Colonies of E1B2-B0015 (AK3) -> E1B2X 3 Colonies of E1B3-B0015 (AK3) -> E1B3X 3 Colonies of E1B4-B0015 (AK3) -> E1B4X
3 Colonies of E21-B0015 (AK3) -> E21X 3 Colonies of E22-B0015 (AK3) -> E22X 3 Colonies of E23-B0015 (AK3) -> E23X 3 Colonies of E24-B0015 (AK3) -> E24X
X= # of colony
--Karl.brune 23:08, 5 September 2009 (UTC)
Colony PCR
TAQ colony PCR was performed. 10µl reaction, standart mix with verfication primer VF2 and VR. Initial Denat: 1min 30sec @94°C 40Cylces Denaturation: 45 sec @94°C Annealing: 45 sec @50°C Elongation: 1min 45sec @ 72°C Return to Denat. Final Elongation: 10min @72°C Infinite storage at 4°C
Colony tubes were moved to fridge after taking template for the PCR
--Karl.brune 23:08, 5 September 2009 (UTC)
