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Team:Chiba/Notebook/Calendar/3 September 2009
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Revision as of 10:59, 8 September 2009
(2_September_2009 <|>4_September_2009)
- 本培養/main culture
10:30 昨日作成したAmp-Cm液体培地30 mLとプレ培養した溶液300 μLを三角フラスコにとり、37℃で振とうした。/we took the liquid medium that we had made yesterday to conical flask 30 mL and added prior culture 300 uL, then started shake culture at 37 degrees Celsius.
- グリスト作成/Making glycerol stock
50%グリセロール300 μLとプレ培養した溶液300 μLを1.5 mLマイクロチューブにとり、Deep Freezerに保存した。/We kept mixture of 300 μL of 50% glycerol and 300 μL of solution which is cultured yesterday in deep freezer.
- トランスファーカーブ作成実験/Making transfer curves
16:00 本培養が終わった溶液を10倍希釈し、48穴Deep wellに入れた。/We put the solution that has completed main culture in 48 deep wells.
17:15 48穴Deep wellを30℃で振とうした。/We shook 48 deep wells at 30 degrees Celsius.
振とうしている間にT=0の時点での試料のOD値および蛍光強度を測定した。/While shaking, we measured OD and GFP fluorescence intensity of sample.
18:15 プレートに試料をとり、OD値と蛍光強度を測定した。
19:15 同様
20:15 同様
※写真は本培養の開始までです。
