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Team:KULeuven/Freezer
From 2009.igem.org
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|||{{kulpart|pSB1A2}}||ech + fcs||Miniprepped Ligatie Jochem (EF4)||48 µl|| 95.7 ng/µl|| September 7 | |||{{kulpart|pSB1A2}}||ech + fcs||Miniprepped Ligatie Jochem (EF4)||48 µl|| 95.7 ng/µl|| September 7 | ||
| + | |- | ||
| + | |{{kulpart|BBa_K238007}}||{{kulpart|pSB1A2}}||sam5||SAM5 purified||23 µl||10.0 ng/µl|| September 7 | ||
|- | |- | ||
|} | |} | ||
Revision as of 12:07, 8 September 2009
Contents |
Blue light receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| MC4100 | / | strain of E.coli | july 24 | |||
| 2260 | / | forward primer for | 42,5µl | 20μM | aug 10 | |
| 2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
| GFP + rbs + terminator | Miniprepped | 100μl | 85,6ng/μl | july 29 | ||
| GFP + rbs + terminator | restricted with EcoRI and XbaI | 20μl | july 29 | |||
| GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | 30μl | 29,3 ng/μl | aug 14 | ||
| kanamycine plasmide backbone | taken from plates | 13μl | aug 11 | |||
| / | Exact blue light promotor | pcr product | 20μl | 123,3 ng/μl | aug 13 | |
| / | constitutive promotor | taken from plates | 13μl | aug 11 | ||
| / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12 | |
| + | LigA: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | aug 13 | ||
| + | Lig B: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | aug 14 | ||
| + | / | Lig A: Exact blue light promotor ligated with in (1,3,7,9) | cut with EcoRI and PstI | 4 x 18μl | aug 24 | |
| + | LigA: Exact blue light promotor ligated with in | Miniprep (4x) | 20μl | aug 18 | ||
| constitutive promotor | miniprep | 10μl | 141,2ng/μl | aug 14 | ||
| (A) | constitutive promotor | restriction digest with EcoRI en SpeI | 20μl | 20,8ng/μl | aug 24 | |
| (B) | constitutive promotor | restriction digest with EcoRI en SpeI | 10μl | 12,8ng/μl | aug 24 | |
| + | LigA (1): Exact blue light promotor ligated with in | Miniprep | 45μl | 110,6ng/μl | aug 31 | |
| + | LigA (2): Exact blue light promotor ligated with in | Miniprep | 43μl | 76,7ng/μl | aug 31 | |
| + | LigA (3): Exact blue light promotor ligated with in | Miniprep | 44μl | 84,8ng/μl | aug 31 | |
| + | LigA (4): Exact blue light promotor ligated with in | Miniprep | 34μl | 31,7ng/μl | aug 31 | |
| pSB1A2 | RBS + GFP + terminator | Miniprep | 45μl | 104,3ng/μl | aug 31 | |
| (A) | pSB1A2 | promotor | Miniprep | 48μl | 238,9ng/μl | sept 2 |
| (B) | pSB1A2 | promotor | Miniprep | 48μl | 248,8ng/μl | sept 2 |
Key/antikey/lock
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | GFP with LVA tag | purified | july 28 | |||
| / | tetR promoter | purified | july 28 |
vanillin synthesis
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| fcs + rbs | purified plasmid (FCS 1) | 13 µl | 32.1 ng/µl | September 1 | ||
| fcs + rbs | purified plasmid (FCS 2) | 28 µl | 67.2 ng/µl | September 1 | ||
| fcs + rbs | purified plasmid (FCS 3) | 28 µl | 82.4 ng/µl | September 1 | ||
| ech + rbs | purified plasmid (ECH 1) | 15 µl | 37.2 ng/µl | September 1 | ||
| ech + rbs | purified plasmid (ECH 2) | 28 µl | 26.9 ng/µl | September 1 | ||
| ech + rbs | purified plasmid (ECH 3) | 28 µl | 28.0 ng/µl | September 1 | ||
| Sam5 + rbs | purified plasmid (SAM5 1) | 19.6 µl | 59.9 ng/µl | September 1 | ||
| Sam5 + rbs | purified plasmid (SAM5 2) | 28 µl | 52.8 ng/µl | September 1 | ||
| Sam5 + rbs | purified plasmid (SAM5 3) | 13 µl | 67.3 ng/µl | September 1 | ||
| Sam8 + rbs | purified plasmid (SAM8 1) | 12 µl | 31.0 ng/µl | September 1 | ||
| Sam8 + rbs | purified plasmid (SAM8 2) | 8 µl | 47.2 ng/µl | September 1 | ||
| Sam8 + rbs | purified plasmid (SAM8 3) | 28 µl | 42.9 ng/µl | September 1 | ||
| sam8 + sam5 | SAMS 1 (from 'mother plate') | 16 µl | 35,4 ng/µl | September 3 | ||
| sam8 + sam5 | SAMS 2 (from 'mother plate') | 19,9 µl | 49,3 ng/µl | September 3 | ||
| sam8 + sam5 | SAMS 3 (from 'mother plate') | 31,9 µl | 62,0 ng/µl | September 3 | ||
| sam8 + sam5 | SAMS 4 (from 'mother plate') | 33,6 µl | 78,3 ng/µl | September 3 | ||
| Terminator | Terminator, B0015 | ±20 µl | 181,4 | September 3 | ||
| sam8 | SAM8 RD 3/9 | 28 µl | 8,2 ng/µl | September 3 | ||
| sam5 | SAM5 RD 3/9 | 28 µl | 1,4 ng/µl | September 3 | ||
| ech | ECH RD 3/9 | 23 µl | 10,1 ng/µl | September 3 | ||
| fcs | FCS RD 3/9 | 4 µl | 5,2 ng/µl | September 3 | ||
| sam8 | SAM8 PCR | µl | 361.6 ng/µl | September 3 | ||
| sam5 | SAM5 PCR | µl | 323.0 ng/µl | September 3 | ||
| ech | ECH PCR | µl | 236.5 ng/µl | September 3 | ||
| fcs | FCS PCR | µl | 321.4 ng/µl | September 3 | ||
| ech + fcs | Ligatie zonder terminator(EFS) | µl | ng/µl | September 3 | ||
| ech + fcs | Ligatie met terminator(EFT) | µl | ng/µl | September 3 | ||
| sam5 + sam8 | Ligatie zonder terminator(58S) | µl | ng/µl | September 4 | ||
| sam5 + sam8 | Ligatie met terminator(58T) | µl | ng/µl | September 4 | ||
| ech + fcs | Miniprepped Ligatie Jochem (EF1) | 48 µl | 115.5 ng/µl | September 7 | ||
| ech + fcs | Miniprepped Ligatie Jochem (EF2) | 48 µl | 105.2 ng/µl | September 7 | ||
| ech + fcs | Miniprepped Ligatie Jochem (EF3) | 48 µl | 95.1 ng/µl | September 7 | ||
| ech + fcs | Miniprepped Ligatie Jochem (EF4) | 48 µl | 95.7 ng/µl | September 7 | ||
| sam5 | SAM5 purified | 23 µl | 10.0 ng/µl | September 7 |
vanillin receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| 2235 | / | forward primer for | 20μM | August 3 | ||
| 2238 | / | reverse primer for | 20μM | August 3 | ||
| 2235 | / | forward primer for | 100μM | August 3 | ||
| 2238 | / | reverse primer for | 100μM | August 3 | ||
| 2242 | / | forward primer for | 20μM | August 3 | ||
| 2243 | / | reverse primer for | 20μM | August 3 | ||
| 2242 | / | forward primer for | 100μM | August 3 | ||
| 2243 | / | reverse primer for | 100μM | August 3 | ||
| 2244 | / | forward primer for | 20μM | August 3 | ||
| 2245 | / | reverse primer for | 20μM | August 3 | ||
| 2244 | / | forward primer for | 100μM | August 3 | ||
| 2245 | / | reverse primer for | 100μM | August 3 | ||
| 2246 | / | forward primer for | 20μM | August 3 | ||
| 2247 | / | reverse primer for | 20μM | August 3 | ||
| 2246 | / | forward primer for | 100μM | August 3 | ||
| 2247 | / | reverse primer for | 100μM | August 3 | ||
| 2288 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2289 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2288 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2289 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2290 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2291 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2290 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2291 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2292 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2293 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2292 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2293 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
| 2294 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
| 2295 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
| 2294 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
| 2295 | / | reverse primer for mutagenesis for | 100μM | August 8 |
miscellaneous
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
| medium constitutieve promoter | july 23 | |||||
| ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/pSB1A2 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/pSB3K3 | Team:KULeuven/BioBricks/BBa J23101 | Team:KULeuven/BioBricks/pSB1A3 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa B0015 | Team:KULeuven/BioBricks/BBa K238008 | Team:KULeuven/BioBricks/BBa K238009 | Team:KULeuven/BioBricks/BBa K238010 | Team:KULeuven/BioBricks/BBa K238011 | Team:KULeuven/BioBricks/BBa J23110
