R-BphP

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Revision as of 11:24, 8 June 2009

The BphP / PpsR system

PpsR1 is a redox sensitive activator. It binds DNA under anaerobic conditions, and forms a tetramer via a disulfide bond. This interaction is ablated in the mutant PpsR1-C429S; meaning that we should be able to mimic an anaerobic system even under aerobic conditions.

PpsR2 is a transcriptional repressor, regulated by BphP.

PpsR1 and PpsR2 bind to TGTN_12ACA possibly arranged in tandem witha 7 base spacer. The affinity for both PpsRs are around 100nM.

BphP (or BrBphP for the Brayrhizobium variant) is sensitive to far-red light (~770nm) and controls PpsR1.

To find

- Define genetic circuit Tú
- Look for exact wavelength for the corresponding light receptor Basile
- Sequences of the genes involved in the pathway (minimal genes to => [http://www.ncbi.nlm.nih.gov/ Pubmed]) Tú

To do

- Contact paper's authors

Found

- Bacterium: Bradyrhizobium can be ordered on [http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=10244&Template=bacteria ATTC]

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+== The BphP / PpsR system ==
+PpsR1 is a redox sensitive activator. It binds DNA under anaerobic conditions, and forms a tetramer via a disulfide bond. This interaction is ablated in the mutant PpsR1-C429S; meaning that we should be able to mimic an anaerobic system even under aerobic conditions.
+PpsR2 is a transcriptional repressor, regulated by BphP.
+PpsR1 and PpsR2 bind to TGTN_12ACA possibly arranged in tandem witha 7 base spacer. The affinity for both PpsRs are around 100nM.
+BphP (or BrBphP for the Brayrhizobium variant) is sensitive to far-red light (~770nm) and controls PpsR1.
 ==To find==
 - Define genetic circuit <b><font size=3><span style="color:green"> Tú </span></font size></b>