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Team:Chiba/Notebook/Calendar/3 September 2009
From 2009.igem.org
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18:15 プレートに試料をとり、OD値と蛍光強度を測定した。 | 18:15 プレートに試料をとり、OD値と蛍光強度を測定した。 | ||
| + | |||
| + | :<table width="200" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="50">cell1 1</td> | ||
| + | :<td width="50">cell2 2</td> | ||
| + | :<td width="50">cell3 3</td> | ||
| + | :<td width="50">cell4 4</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>cell5 5</td> | ||
| + | :<td>cell6 6</td> | ||
| + | :<td>cell7 7</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
19:15 同様 | 19:15 同様 | ||
| + | |||
| + | :<table width="200" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="50">cell1 1</td> | ||
| + | :<td width="50">cell2 2</td> | ||
| + | :<td width="50">cell3 3</td> | ||
| + | :<td width="50">cell4 4</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>cell5 5</td> | ||
| + | :<td>cell6 6</td> | ||
| + | :<td>cell7 7</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
20:15 同様 | 20:15 同様 | ||
| + | :<table width="200" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="50">cell1 1</td> | ||
| + | :<td width="50">cell2 2</td> | ||
| + | :<td width="50">cell3 3</td> | ||
| + | :<td width="50">cell4 4</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>cell5 5</td> | ||
| + | :<td>cell6 6</td> | ||
| + | :<td>cell7 7</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
| + | |||
| + | 21:15 | ||
| + | |||
| + | :<table width="200" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | :<td width="50">cell1 1</td> | ||
| + | :<td width="50">cell2 2</td> | ||
| + | :<td width="50">cell3 3</td> | ||
| + | :<td width="50">cell4 4</td> | ||
| + | :</tr> | ||
| + | :<tr> | ||
| + | :<td>cell5 5</td> | ||
| + | :<td>cell6 6</td> | ||
| + | :<td>cell7 7</td> | ||
| + | :</tr> | ||
| + | :</table> | ||
Revision as of 06:43, 14 September 2009
(2_September_2009 <|>4_September_2009)
- 本培養/main culture
10:30 昨日作成したAmp-Cm液体培地30 mLとプレ培養した溶液300 μLを三角フラスコにとり、37℃で振とうした。/we took the liquid medium that we had made yesterday to conical flask 30 mL and added prior culture 300 uL, then started shake culture at 37 degrees Celsius.
- グリスト作成/Making glycerol stock
50%グリセロール300 μLとプレ培養した溶液300 μLを1.5 mLマイクロチューブにとり、Deep Freezerに保存した。/We kept mixture of 300 μL of 50% glycerol and 300 μL of solution which is cultured yesterday in deep freezer.
- トランスファーカーブ作成実験/Making transfer curves
16:00 本培養が終わった溶液を10倍希釈し、48穴Deep wellに入れた。/We put the solution that has completed main culture in 48 deep wells.
17:15 48穴Deep wellを30℃で振とうした。/We shook 48 deep wells at 30 degrees Celsius.
振とうしている間にT=0の時点での試料のOD値および蛍光強度を測定した。/While shaking, we measured OD and GFP fluorescence intensity of sample.
18:15 プレートに試料をとり、OD値と蛍光強度を測定した。
cell1 1 cell2 2 cell3 3 cell4 4 cell5 5 cell6 6 cell7 7
19:15 同様
cell1 1 cell2 2 cell3 3 cell4 4 cell5 5 cell6 6 cell7 7
20:15 同様
cell1 1 cell2 2 cell3 3 cell4 4 cell5 5 cell6 6 cell7 7
21:15
cell1 1 cell2 2 cell3 3 cell4 4 cell5 5 cell6 6 cell7 7
※写真は本培養の開始までです。
