Template:Team:KULeuven/9 September 2009/VanillinProduction

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(Difference between revisions)
(New page: * Restriction digest on Terminator * Growth on SAMS plates (woohoo!) * Transfered 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate)
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* Growth on SAMS plates (woohoo!)
* Growth on SAMS plates (woohoo!)
* Transfered 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate
* Transfered 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate
 +
* Gel electrophorese of all the 8 EF restrictrion digests (2 per colony)
 +
** Cut and purified EF1 and EF3
 +
{| border ="1" align="center"
 +
!| part || concentration || 260/280 || 260/230
 +
|- align="center"
 +
| EF1 || 5,2 || 1,43 ||
 +
|- align="center"
 +
| EF3 || 4,3 || 1,45 ||
 +
|-
 +
|}

Revision as of 14:40, 9 September 2009

  • Restriction digest on Terminator
  • Growth on SAMS plates (woohoo!)
  • Transfered 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate
  • Gel electrophorese of all the 8 EF restrictrion digests (2 per colony)
    • Cut and purified EF1 and EF3
part concentration 260/280 260/230
EF1 5,2 1,43
EF3 4,3 1,45
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/9_September_2009/VanillinProduction"