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Team:Warsaw/Calendar-Main/2 September 2009
From 2009.igem.org
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<h4>Marcin</h4> | <h4>Marcin</h4> | ||
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| - | <p>Task 1:</p><ul><li>Restriction digest of followed costruct: <a href="http://partsregistry.org/Part: | + | <p>Task 1:</p><ul><li>Restriction digest of followed costruct: <a href="http://partsregistry.org/Part:BBa_K177037"><span style="color: black">BBa_K177037</a></span> from the <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li></ul> |
<p>Methods:</p> | <p>Methods:</p> | ||
<ul><li>Reaction mixture composition:</li><pre> | <ul><li>Reaction mixture composition:</li><pre> | ||
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<p>Task 2:</p> | <p>Task 2:</p> | ||
| - | <ul><li>Prepare the bacterial cultures for isolation of on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid containing <a href="http://partsregistry.org/Part: | + | <ul><li>Prepare the bacterial cultures for isolation of on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid containing <a href="http://partsregistry.org/Part:BBa_K177035"><span style="color: black">BBa_K177035</a></span> |
</ul> | </ul> | ||
Latest revision as of 09:24, 11 September 2009
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Assembly of endosomal detection operon
Marcin
Task 1:
- Restriction digest of followed costruct: BBa_K177037 from the pSB1A3 plasmid
Methods:
- Reaction mixture composition:
1. μl purified plasmid DNA product 1 μl XbaI (Fermentas) 1 μl PstI (Fermentas) 2 μl Buffer Tango (Fermentas) 15 μl MQ water
Results
- All isolated plasmids have no insert
Task 2:
- Prepare the bacterial cultures for isolation of on pSB1A3 plasmid containing BBa_K177035
Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Preparation of PCR reaction with L.monocytogenes st. EGD-e genome DNA
- Separation of PCR products on the 0,8% agarose gel
- Acquiring the pure DNA sample from PCR reaction with Clean-Up kit
Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Liquid cultures establishment
Methods:
- Appropriate colonies (both of them) were transferred to a new plate containing ampicilin
- Liquid cultures were established in 5 ml LB medium supplemented with ampicilin and incubated overnight with areation
Cloning of the cro-box into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Liquid cultures establishment
Methods:
- Appropriate colonies (all 7 of them) were transferred to a new plate containing ampicilin
- Liquid cultures were established in 5 ml LB medium supplemented with ampicilin and incubated overnight with areation
Ania
Tasks:
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