Team:Imperial College London/M3

=Overview= ==What:== The aim of Module 3 is to cut any genetic material present within the bacterium, whilst leaving the cell membrane intact, so as to maintain the protective capsule around our drug protein. The destroyed genetic material ensures that E. coli will be non-pathogenic to consumers. We will have a floating sack of protein contained within the secreted capsule. ==Why== The E.ncapsulator requires the E. coli to be dead upon ingestion. This will prevent any transfer of genetic material between the bacterium and any gut microflora present, thereby avoiding any unexpected pathogenic effects. This is also especially important if the E.ncapsulator is to attain public acceptance, due to concerns over genetically modified organisms. ==When== The thermally induced killing mechanism will only be triggered once encapsulation is complete. ==How== Restriction enzymes found within bacteria and act as defense mechanisms against invading viruses. They work by recognising a certain DNA sequence of a few bases, and then cleaving the DNA strand. The E.ncapsulator is engineered to manufacture the restriction enzymes DpnII and TaqI when triggered, and these will cleave the genetic material within into fragments - thereby killing the cell. As a protective mechanism against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Dam methylase protection system. This methylates DNA, which means that only high levels of restriction enzyme (ie. upon trigger) will cleave the DNA. is induced, rendering the bacterium no more than an inanimate shell containing our protein drug of choice. {{Imperial/09/TemplateBottom}}