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Team:KULeuven/Design/Blue Light Receptor
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| - | The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm. | + | The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain ''E. coli'' strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm. |
| - | We designed the {{kulpart|BBa_K238002}} part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the E. coli- strain MC4100. After mutating out possible restriction sites, the blr promoter part ({{kulpart|BBa_K238000}}) was added to the registry. | + | We designed the {{kulpart|BBa_K238002}} part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the ''E. coli''- strain MC4100. After mutating out possible restriction sites, the ''blr'' promoter part ({{kulpart|BBa_K238000}}) was added to the registry. |
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Revision as of 12:56, 14 September 2009
The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm.
We designed the part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the E. coli- strain MC4100. After mutating out possible restriction sites, the blr promoter part () was added to the registry.
