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Template:Team:KULeuven/13 August 2009/VanillinProduction
From 2009.igem.org
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# Restriction digest on ''ech'' and ''sam8'' | # Restriction digest on ''ech'' and ''sam8'' | ||
| - | # | + | # Gel electrophoresis on ''ech'' and ''sam8'' |
# New electrocompetent cells were used for the electroporation with the old ligations | # New electrocompetent cells were used for the electroporation with the old ligations | ||
#* Lig A -> ''sam5 + sam8'' | #* Lig A -> ''sam5 + sam8'' | ||
Latest revision as of 10:01, 14 September 2009
- Plated COMT (+RBS) from the registry
- Growing overnight in 37°C
- Plated ech
- From newly made liquid culture
- Miniprep of ech and sam8
- Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| ech | 72,7 | 1,94 | |
| sam8 | 66,2 | 2,00 |
- Restriction digest on ech and sam8
- Gel electrophoresis on ech and sam8
- New electrocompetent cells were used for the electroporation with the old ligations
- Lig A -> sam5 + sam8
- Lig C -> ech + fcs
- Note: there was no spark
- Plating of the electroporated cells
- 200 μl per plate twice
- 600 μl left of A and C
