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Template:Team:KULeuven/21 August 2009/VanillinProduction
From 2009.igem.org
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| - | * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. | + | * <u>Test 2</u>: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. |
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ''ech''-plasmid. The signals do not correspond with an EF-ligation product. | However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ''ech''-plasmid. The signals do not correspond with an EF-ligation product. | ||
Latest revision as of 11:39, 14 September 2009
- Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ech-plasmid. The signals do not correspond with an EF-ligation product.
- The DNA was purified from the gel
Nanodrop results:
| Part | ng/µl | 260/280 | |
|---|---|---|---|
| sam8 | 2,5 | 1,14 | |
| sam5 | 7,9 | 1,60 | |
| ech | 5,0 | 1,38 | |
| fcs | 3,4 | 0,93 |
- sam8 and sam5; fcs and ech were ligated
Used volumes:
| Part | µl | |
|---|---|---|
| sam8 | 34 | |
| sam5 | 6,3 | |
| ech | 10,0 | |
| fcs | 35,8 |
