Team:Wash U/Calendar-Home/10 June 2009

(Difference between revisions)

Revision as of 20:58, 19 June 2009

Estimated 5-15ng of DNA per well.

Using 5ul of DNA (diluted 1:16 in TE)

Allow the DNA and competent cells to sit on ice for 30 minutes

Heat shock at 42ºC for 60 sec in water bath.

Recover on ice for 5 min.

Incubate at 37ºC for 2 hr while the tubes are rotating.

Plate 250µL on an LB plate with the appropriate antibiotic.

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