Team:Alberta/Project/Recombineering

From 2009.igem.org

(Difference between revisions)
Line 48: Line 48:
     </td>
     </td>
   </tr>
   </tr>
 +
 +
<tr>
 +
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
 +
    <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
 +
    <div class="Outreach">
 +
    <div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;">
 +
    <h1>What is Recombineering?</h1>
 +
 +
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> -->
 +
<div align="justify">
 +
 +
<font size="2">
</table>
</table>
</div>
</div>
</HTML>
</HTML>

Revision as of 20:53, 17 September 2009

University of Alberta - BioBytes










































































































What is Recombineering?

Recombineering refers to the strategic use of recombination in vivo in order to reach a defined goal. In the case of BioBytes, a method is required to target the final construct to insertion at a specific place on the E. coli chromosome.

To do this successfully, three components must be taken into account:

- There must be a system for targeting the construct to a specific site for insertion

- Activation of the recombination system must be under experimenter control

- It must be possible to select for and verify colonies in which the insertion was successful

What is Recombineering?