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Team:HKUST/Protocols
From 2009.igem.org
(Difference between revisions)
| Line 57: | Line 57: | ||
<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Yeast_genomic_DNA_extraction"> Yeast genomic DNA | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/Yeast_genomic_DNA_extraction"> Yeast genomic DNA | ||
extraction</a></li> | extraction</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/Western_blotting"> Western blotting</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/GFP_testing"> GFP testing</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/Biological_test"> Biological test</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 13:23, 21 September 2009
Welcome
- Agarose gel preparation and gel electrophoresis
- PCR (Taq, Vent)
- PCR product clean-up
- Enzyme digestion (vector, insert)
- Plasmid DNA extraction
- Gel cutting
- Gel extraction
- Ethanol precipitation
- Ligation
- Transformation to E.coli
- Transformation to yeast
- Cell lysis
- Yeast genomic DNA extraction
- Western blotting
- GFP testing
- Biological test
Products
iGEM 2009
