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Team:Illinois/SgrS
From 2009.igem.org
(Difference between revisions)
(New page: {{UIUC09Temp}} {{Illinois}} == '''SgrS Target-GFP Fusion''' == '''People in Group:''' Francis Lee, Harsh Shah, Dave Korenchan '''Purpose:''' We want to fuse the SgrS target sequence fro...) |
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'''People in Group:''' Francis Lee, Harsh Shah, Dave Korenchan | '''People in Group:''' Francis Lee, Harsh Shah, Dave Korenchan | ||
| - | '''Purpose:''' We want to fuse the SgrS target sequence from the PtsG gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the | + | '''Purpose:''' We want to fuse the SgrS target sequence from the PtsG gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the SgrS gene. We expect to see translational repression of GFP by the small RNA SgrS. |
'''Protocol(s) Used:''' (links to protocols page) | '''Protocol(s) Used:''' (links to protocols page) | ||
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'''Recipe(s) Used:''' | '''Recipe(s) Used:''' | ||
| - | '''Primers Used:''' | + | '''Primers Used:''' |
== '''June 12''' == | == '''June 12''' == | ||
Journal entry text goes here. | Journal entry text goes here. | ||
Revision as of 20:55, 11 June 2009
SgrS Target-GFP Fusion
People in Group: Francis Lee, Harsh Shah, Dave Korenchan
Purpose: We want to fuse the SgrS target sequence from the PtsG gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the SgrS gene. We expect to see translational repression of GFP by the small RNA SgrS.
Protocol(s) Used: (links to protocols page)
Recipe(s) Used:
Primers Used:
June 12
Journal entry text goes here.
