Team:Chiba/Notebook/Calendar/18 September 2009

From 2009.igem.org

(Difference between revisions)
(→Las Check)
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Sender:Receiver=0.8 mL:0.8 mL
Sender:Receiver=0.8 mL:0.8 mL
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(1):1 + 4 ---LasI/LasR-GFP
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(2):1 + 3 ---LasI/LuxR-GFP
 +
 +
(3):1 + 5 ---LasI/LuxR-RFP
 +
 +
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(4):2 + 3 ---LuxI
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(5):2 + 4
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(6):2 + 5
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(7):3 + flesh LB
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(8):4 + flesh LB
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 +
(9):5 + flesh LB
== Lab Notebook ==
== Lab Notebook ==
[[Image:Chiba-Labwork-17Sep09.jpg|200px]] [[Image:Chiba-Labwork-18Sep09.jpg|200px]]
[[Image:Chiba-Labwork-17Sep09.jpg|200px]] [[Image:Chiba-Labwork-18Sep09.jpg|200px]]
[[Image:Chiba-Labwork-17Sep09-5.jpg|200px]] [[Image:Chiba-Labwork-17Sep09-2.jpg|200px]]
[[Image:Chiba-Labwork-17Sep09-5.jpg|200px]] [[Image:Chiba-Labwork-17Sep09-2.jpg|200px]]

Revision as of 06:44, 23 September 2009

>Go to the Notebook page

(17_September_2009 <|>19_September_2009)

LuxR Mutants Sequence

Purification of DNA

  • isopropanol precipitation and analysis
  1. add isopropanol(75%) 50 uL to PCR solution made yesterday
  2. VORTEX and centrifugation(14000 rpm 10 min)
  3. put off the supernatant fluid
  4. add isopropanol(75%) 50 uL
  5. centrifugation(14000 rpm 10 min)
  6. put off the supernatant fluid
  7. drying 60 degrees C 15min
  8. add ddH2O 20 uL and VORTEX
  9. sequence analysis

Las Check

Culture Mix

  1. K084007:LasI
  2. K084012:LuxI
  3. T9002:LuxR-GFP
  4. K091134:LasR-GFP
  5. I731012:LuxR-RFP


Sender:Receiver=0.8 mL:0.8 mL

(1):1 + 4 ---LasI/LasR-GFP

(2):1 + 3 ---LasI/LuxR-GFP

(3):1 + 5 ---LasI/LuxR-RFP


(4):2 + 3 ---LuxI

(5):2 + 4

(6):2 + 5


(7):3 + flesh LB

(8):4 + flesh LB

(9):5 + flesh LB

Lab Notebook

Chiba-Labwork-17Sep09.jpg Chiba-Labwork-18Sep09.jpg Chiba-Labwork-17Sep09-5.jpg Chiba-Labwork-17Sep09-2.jpg

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/18_September_2009"